货号：15232010

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：2000UN

目录价：￥492.00

市场价格：￥442.80

会员价格：￥442.80

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 5´-GGTAC↓C-3´ 3´-C↑CATGG-5´Important: Invitrogen restriction enzymes now come with Universal Buffers L, M, H, K, or T (+BSA) instead of REact® Buffers 1-8. Functionally, the enzymes are the same and have been validated for the recommended buffer. Please contact our technical services department immediately if you have questions or concerns.

Concentration	10 U/ µL
Contents	Kpn I 10X Buffer L (1mL) 10X Loading Buffer (1mL)
Reaction Temperature	37°C
Reaction Mixture	Kpn I 1 µL 10X Buffer L 2 µL Substrate DNA <1 µg Sterile water to 20 µL
Heat inactivation	The enzyme cannot be heat inactivated. To inactivate this enzyme, purify DNA using silica-column based DNA purification kits such as the PureLink™ HiPure Miniprep Kit or perform a phenol/chloroform extraction followed by ethanol precipitation.
Star Activity	Unrelated site may be cut under conditions of low ionic strength, high enzyme concentration, or glycerol concentration >5%.
Effect of DNA Methylation	Enzyme activity is not affected by CG methylase.
Unit Definition	One unit is the amount of enzyme required to completely digest 1 µg of λDNA in 50 µL of the reaction mixture in one hour at 37°C.
10X Buffer L	100 mM Tris-HCl, pH 7.5 100 mM MgCl2 10 mM Dithiothreitol
Source	Klebsiella pneumoniae

Relative Activity in Universal Reaction Buffers

Buffer	L	M	H	K	T(+BSA)
Relative Activity (%)	100	60	<20	<20	100*

* weak star activity is detected For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use. 规格

Enzyme:	Kpn I
Product Size:	2,000 units
Concentration:	10 U⁄µl
Star Activity:	May exhibit star activity
Compatible Buffer:	10X Buffer L
Methylation Sensitivity:	Not dam methylation-sensitive,Not CpG methylation-sensitive,Not dcm methylation-sensitive
Optimal Reaction Temperature:	37° C
Sensitive to Heat Inactivation:	No (Resistant to Heat Inactivation)
Shipping Condition:	Approved for shipment on Wet or Dry Ice

内容及储存 Storage Buffer10 mM Tris-HCl, pH 7.5100 mM KCl0.1 mM EDTA1 mM Dithiothreitol (DTT)0.15% Triton® X-1000.01% BSA50% (v⁄v) glycerolLoading Buffer1% SDS50% glycerol0.05% Bromophenol blueStore enzyme and buffer at -20°C, and loading buffer at RT.