货号：15224025

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：500 UN

目录价：￥1779.00

市场价格：￥1601.10

会员价格：￥1601.10

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 T4 DNA Ligase catalyzes the formation of phosphodiester bonds in the presence of ATP between double-stranded DNAs with 3´ hydroxyl and 5´ phosphate termini. The unique T4 DNA Ligase buffer optimizes ligation, which can be performed in 5 minutes. Single-stranded nucleic acids are not substrates for this enzyme.ApplicationsCloning (blunt-end or cohesive-end ligation) and adding linkers or adapters to blunt-ended DNASourcePurified from E. coli lambda lysogen NM989Performance and quality testingEndodeoxyribonuclease, 3´ and 5´ exodeoxyribonuclease assays; ligation efficiency testedUnit definitionOne unit catalyzes the exchange of 1 nmol ³²P-labeled pyrophosphate into ATP in 20 min at 37°C. One unit is equal to approximately 300 cohesive-end ligation units.Unit reaction conditions66 mM Tris-HCl (pH 7.6), 6.6 mM MgCl₂, 10 mM DTT, 66 µM ATP, 3.3 µM ³²P-labeled pyrophosphate, and enzyme in 0.1 mL for 20 min at 37°C. 规格

Enzyme:	Ligase
Product Size:	500 units
Concentration:	1 U⁄µl
Shipping Condition:	Dry Ice

内容及储存 T4 DNA Ligase is supplied with a vial of 5X reaction buffer [250 mM Tris-HCl (pH 7.6), 50 mM MgCl₂ , 5 mM ATP, 5 mM DTT, 25% (w/v) polyethylene glycol-8000]. Store at -20°C.