货号：15201049

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：10000 UN

目录价：￥1697.00

市场价格：￥1527.30

会员价格：￥1527.30

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 5´-G↓GATCC-3´ 3´-CCTAG↑G-5´Important: Invitrogen restriction enzymes now come with Universal Buffers L, M, H, K, or T (+BSA) instead of REact® Buffers 1-8. Functionally, the enzymes are the same and have been validated for the recommended buffer. Please contact our technical services department immediately if you have questions or concerns.

Concentration	50 U/ µL
Contents	BamH I 10X Buffer K (1 mL) 10X Loading Buffer (1 mL)
Reaction Temperature	30°C
Reaction Mixture	BamH I 1 µL 10X Buffer K 2 µL Substrate DNA < 1 µg Sterile water to 20 µL
Heat inactivation	Enzyme inactivated by heating at 65°C for 20 minutes.
Star Activity	Unrelated site may often be cut in the presence of high concentrations of glycerol or Mn2+ and at low ionic strength.
Effect of DNA Methylation	Enzyme activity is not affected by CG methylase, dam methylase, and dcm methylase.
Unit Definition	One unit is the amount of enzyme required to completely digest 1 µg of λDNA in 50 µL of the reaction mixture in one hour at 30°C.
10X Buffer K	200 mM Tris-HCl, pH 8.5 100 mM MgCl2 10 mM Dithiothreitol 1,000 mM KCl
Source	Bacillus amyloliquefaciens H

Relative Activity in Universal Reaction Buffers

Buffer	L	M	H	K	T(+BSA)
Relative Activity (%)	<20*	<20	40	100	<20*

* weak star activity is detected For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use. 规格

Enzyme:	BamH I
Product Size:	10,000 units
Concentration:	50 U⁄µl
Star Activity:	May exhibit star activity
Compatible Buffer:	10X Buffer K
Methylation Sensitivity:	Not dam methylation-sensitive,Not CpG methylation-sensitive,Not dcm methylation-sensitive
Optimal Reaction Temperature:	37° C
Sensitive to Heat Inactivation:	Yes (Heat-Inactivatable)
Shipping Condition:	Approved for shipment on Wet or Dry Ice

内容及储存 Storage Buffer10 mM Tris-HCl, pH 7.5400 mM KCl0.1 mM EDTA1 mM DTT0.15% Triton® X-1000.01% BSA50% (v⁄v) glycerolLoading Buffer1% SDS50% glycerol0.05% Bromophenol blueStore enzyme and buffer at -20°C, and loading buffer at RT.