货号:12580049
品牌:ThermoFisher /Invitrogen/ Gibco/ ABI
规格:5X96WELLPLATES
目录价:¥9258.00
市场价格:¥8332.20
会员价格:¥8332.20
金山科研平台,产品价格货期咨询微信:jinshanbio
描述
Platinum® Taq DNA Polymerase is ideal for automatic "hot-start" amplification of DNA fragments with improved specificity over Taq DNA polymerase. It is derived from recombinant Taq DNA polymerase by binding of a thermolabile inhibitor containing monoclonal antibodies to Taq DNA polymerase. During the initial denaturation step of PCR, the inhibitor is denatured and active Taq DNA polymerase is released into the reaction.With Platinum® Taq you'll get:• Improved specificity and yields of PCR compared to Taq DNA polymerase without "hot-start"• PCR products up to 5 kb in size• Room temperature reaction assembly (Figure 1)Platinum® Blue PCR SuperMix is a PCR master mix containing Platinum® Taq DNA Polymerase, dNTPs, premixed loading/tracking dye, buffer, magnesium and salts. The blue tracking buffer will not inhibit your PCR performance. After PCR cycling, simply load your PCR product directly onto an agarose gel.Platinum® Taq PCRx DNA Polymerase is Platinum® Taq DNA Polymerase supplied with the PCRx Enhancer System for optimizing PCR of problematic and/or GC-rich templates. 10X PCRx Enhancer Solution with 10X PCRx Amplification Buffer offers higher primer specificity, broader magnesium concentration, broader annealing temperature, and improved thermostability of Taq DNA polymerase.Applications: Amplification of DNA from complex genomic, viral, and plasmid templates; and RT-PCR (1).Unit Definition: One unit of Platinum® Taq DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of deoxyribonu-cleotide into DNA in 30 min. at 74°C.
规格
Polymerase: |
Platinum® Taq DNA Polymerase |
Hot Start: |
Built-In Hot Start |
Fidelity (vs. Taq): |
1 X |
GC-Rich PCR Performance: |
Low |
Reaction Format: |
SuperMix or Master Mix |
Product Overhang: |
3'-A |
Reaction Speed: |
Standard |
Final Product Size(s): |
5 kb or less |
Product Size: |
5 plates |
Exonuclease Activity: |
5' - 3' |
Shipping Condition: |
Dry Ice |
内容及储存
Platinum®
Taq is supplied with 10X PCR buffer [200 mM Tris-HCl (pH 8.4), 500 mM KCl], and 50 mM MgCl 2 . Platinum®
Taq PCR
x DNA Polymerase is supplied with 10X PCR buffer, 50 mM MgCl 2 , 50 mM MgS0 4 , 10X PCR
x Enhancer Solution, and 10X PCR
x Amplification Buffer. SuperMixes are supplied in a 1.1X concentration and contain Platinum®
Taq , dNTPs, MgS0 4 ,and buffer. Platinum® Blue PCR SuperMix also contains loading/tracking dye. Store at -20°C. Guaranteed stable for 6 months when properly stored.
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