货号：12574030

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：25 REACTIONS

目录价：￥4284.00

市场价格：￥3855.60

会员价格：￥3855.60

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 The SuperScript® III One-Step RT-PCR System with Platinum® Taq DNA Polymerase offers the highest sensitivity available from Life Technologies for end point detection. This system combines the high thermostability of SuperScript® III RT with the specificity of Platinum® Taq DNA Polymerase High Fidelity to increase priming specificity, generate higher product yields, and identify a larger range of targets. The one-step format enables easy analysis of gene expression or detection of rare or viral RNA.• Sensitivity - Routine detection down to 1 pg total RNA• Convenience - Single-tube format for speed, convenience, and less reaction-to-reaction variability. Compatible with multiplexing• Specificity - Uses SuperScript® III RT for cDNA synthesis up to 55°C, for more specific priming with gene specific primers• Amplicon Size - Detection of targets from 300 bp to 10 kb for greater flexibility (Figure 1)High-Fidelity PCR Amplification Platinum® Taq DNA Polymerase High Fidelity is ideal for the amplification of DNA fragments, when high yields and robust amplification are required. Fidelity is maximized through the use of a mixture of Platinum® Taq DNA Polymerase and the proofreading (3’-5’ exonuclease activity) enzyme Pyrococcus species GB-D polymerase. PCR specificity is improved with the incorporation of Platinum® automatic "hot-start" technology. For Research Use Only. Not for human or animal therapeutic or diagnostic use. 规格

PCR Method:	1-Step RT-PCR
Reverse Transcriptase:	SuperScript® III
Polymerase:	Platinum® Taq DNA Polymerase High Fidelity
Hot Start:	Built-In Hot Start
Sample Type (General):	RNA
Product Size:	25 reactions
Detection Method:	Gel Electrophoresis
Shipping Condition:	Dry Ice
Final Product Size(s):	10 kb or less
Primer-Probe Compatibility:	Unlabeled Oligos

内容及储存 Components: • 25 µL SuperScript® III RT/Platinum® Taq High Fidelity Enzyme Mix • 1 mL 2X Reaction Mix (containing 0.4 mM of each dNTP, 2.4 mM MgSO₄) • 500 µL 5 mM Magnesium Sulfate Store all components at -30°C to -10°C.