货号：12532016

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：100 REACTIONS

目录价：￥2891.00

市场价格：￥2601.90

会员价格：￥2601.90

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 Platinum® PCR SuperMix High Fidelity 是即用型混合物，包含 DNA 聚合酶、盐、镁和 dNTP，用于高效 PCR 扩增。 只需加入模板和引物，将设置时间减少一半（图 1）。Platinum® PCR SuperMix High Fidelity 用于高特异性、高保真 DNA 扩增应用，比如克隆或突变。 Taq DNA 聚合酶和具有校正功能的火球菌属菌种 GB-D 聚合酶的混合物可提供高保真度。 通过抗-Taq 抗体实现高特异性，从而实现自动"热启动" PCR。 这种热启动功能可增强特异性和产量，同时还可以在室温下准备反应体系。 Platinum® PCR SuperMix High Fidelity 可以：-实现比 Taq DNA 聚合酶高出六倍以上的保真度-扩增长达 15 kb 的片段-产生平末端和 3-A' 末端混合的产物；但是，大部分末端具有 3-A’ 突出-最大程度地减少 PCR 优化 规格

Polymerase:	Platinum® Taq DNA Polymerase High Fidelity
Hot Start:	Built-In Hot Start
Fidelity (vs. Taq):	6 X
GC-Rich PCR Performance:	Low
Reaction Format:	SuperMix or Master Mix
Product Overhang:	Mixed
Reaction Speed:	Standard
Final Product Size(s):	15 kb or less
Product Size:	100 reactions
Exonuclease Activity:	5' - 3'
Shipping Condition:	Approved for shipment on Wet or Dry Ice

内容及储存 Platinum® PCR SuperMix High Fidelity is supplied at 1.1X concentration to allow approximately 10% of the final reaction volume to be used for the addition of primer and template solutions. Reagents sufficient for 100 amplification reactions of 50 µl each are provided. Platinum® PCR SuperMix High Fidelity Components: 22 U/ml complexed recombinant Taq DNA polymerase, Pyrococcus species GB-D thermostable polymerase, and Platinum® Taq Antibody; 66 mM Tris-SO4 (pH 8.9); 19.8 mM (NH4)2SO4; 2.4 mM MgSO4; 220 µM dNTPs; and stabilizers. 100 rxns: 4 x 1.125 ml Store at -20°C in a non-frost-free freezer.