货号:12355036
品牌:ThermoFisher /Invitrogen/ Gibco/ ABI
规格:500 RXNS
目录价:¥6384.00
市场价格:¥5745.60
会员价格:¥5745.60
金山科研平台,产品价格货期咨询微信:jinshanbio
描述
Pfx50™ DNA Polymerase is a designer enzyme derived from the archaean Thermococcus zilligii. In addition to 3´ to 5´ proofreading ability, Pfx50™ polymerase is fused to an accessory protein for enhanced fidelity and processivity. This unique combination makes the Pfx50™ enzyme ideal for applications that require the highest fidelity and PCR success the first time. With Pfx50™ DNA Polymerase, you will:• Achieve ultra low error rates—50 times better than Taq, without compromising PCR robustness• Save time and money—highest fidelity means youll sequence fewer clones to obtain error-free constructs• Produce results quickly and easily—robust performance with extremely short extension holdsDownstream compatibility with popular cloning techniquesPfx50™ DNA Polymerase produces blunt-ended PCR products for compatibility with Directional TOPO® Expression Kits and Zero Blunt® TOPO® PCR Cloning Kits.Unit Definition:One unit of Pfx50™ DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-insoluble material in 30 min at 74°C.
规格
Polymerase: |
Pfx50™ DNA Polymerase |
Hot Start: |
No |
Fidelity (vs. Taq): |
50 X |
GC-Rich PCR Performance: |
Low |
Reaction Format: |
Separate Components |
Product Overhang: |
Blunt |
Reaction Speed: |
Standard |
Final Product Size(s): |
4 kb or less |
Product Size: |
500 reactions |
Exonuclease Activity: |
3' - 5' |
Shipping Condition: |
Dry Ice |
内容及储存
Pfx50™
DNA Polymerase is supplied with a vial of 10X
Pfx50™
PCR amplification buffer contains 1.2 mM MgSO
4 and a vial of 50 mM MgSO
4 for further optimization. Store at -20°C. Guaranteed stable for 6 months when properly stored.
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