货号:12341020
品牌:ThermoFisher /Invitrogen/ Gibco/ ABI
规格:1000 RX
目录价:¥17708.00
市场价格:¥15937.20
会员价格:¥15937.20
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描述
AccuPrime™ SuperMix II is a ready-to-use mixture of DNA polymerase, accesory proteins, salts, magnesium, and dNTPs for efficient PCR amplification. All you have to do is add template and primers, reducing set-up time by half (Figure 1).For accurate PCR performance, AccuPrime™ SuperMix II sets the standard. You'll amplify your DNA targets cycle after cycle, eliminating troubleshooting and rework. In addition to a powerful DNA polymerase, AccuPrime™ SuperMix I includes Platinum® anti-DNA polymerase antibodies that inhibit polymerase activity, providing an automatic hot-start, and a thermal stable accessory protein that enhances specific primer-template hybridization during every cycle of PCR (Figure 1). This unique combination allows for success, even under previously suboptimal conditions.AccuPrime™ SuperMix II is designed for amplification of genomic DNA amplicons, plasmid DNA, or cDNA templates up to 4kb in length. Alternatively, AccuPrime™ SuperMix I is optimized to amplify a tighter range of targets less than 200bp in length.
规格
Polymerase: |
AccuPrime™ Taq DNA Polymerase |
Hot Start: |
Built-In Hot Start |
Fidelity (vs. Taq): |
2 X |
GC-Rich PCR Performance: |
Low |
Reaction Format: |
SuperMix or Master Mix |
Product Overhang: |
3'-A |
Reaction Speed: |
Standard |
Final Product Size(s): |
4 kb or less |
Product Size: |
1,000 reactions |
Exonuclease Activity: |
5' - 3' |
Shipping Condition: |
Dry Ice |
内容及储存
AccuPrime™ SuperMix II is supplied at 2X concentration to allow approximately 50% of the final reaction volume to be used for the addition of primer and template solutions. Reagents sufficient for 1,000 amplification reactions of 25 ?l each are provided.AccuPrime™ SuperMix II Components:40 mM Tris-HCl (pH 8.4); 100 mM KCl; 3 mM MgCl2, 400 ?M each dGTP, dATP, dTTP, dCTP; AccuPrime™ Taq DNA Polymerase; thermostable AccuPrime™ protein; stabilizers.1,000 rxns: 1 × 12.5 mlSore at -20°C in a non-frost-free freezer.
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