货号：12338002

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：20L, Bag

目录价：￥40467.00

市场价格：￥36420.30

会员价格：￥36420.30

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 FreeStyle™ 293 Expression Medium is an animal origin–free, chemically defined, protein-free medium specifically developed to support the growth and transfection of 293-F cells in suspension without adaptation. The medium is complete and ready-to-use, with no supplementation required. Gibco® FreeStyle™ 293 Expression Medium features:• Complete and ready-to-use format containing GlutaMAX™ supplement• Ability to support suspension growth and transfection of 293-F cells• Animal origin–free, protein-free and chemically defined formulation• Easy use with the FreeStyle™ 293 Expression systems• Scalability in spinner flasks and bioreactorsComplete and ready-to-use format containing GlutaMAX™ supplementGibco® FreeStyle™ 293 Expression Medium is ready to use. It does not require the addition of serum, glutamine, or surfactants. Gibco® FreeStyle™ 293 Expression Medium contains GlutaMAX™ supplement to minimize toxic ammonia build-up and improve cell viability and growth in an easy-to-use format.Ability to support suspension growth and transfection of 293-F cellsGibco® 293-F, 293-H, and FreeStyle™ 293-F cells in suspension can be cultured directly into Gibco® FreeStyle™ 293 Expression Medium without adaptation. Cells adapted to other commercially available serum-free media can be subcultured directly into Gibco® FreeStyle™ 293 Expression Medium, usually without any further adaptation. Cells usually require adaptation from serum-containing formulations. Cells cultured in Gibco® FreeStyle™ 293 Expression Medium can be transfected using lipid based transfection reagents, such as 293fectin™ reagent and Freestyle™ MAX reagent.Animal origin–free, protein-free and chemically defined formulationGibco® FreeStyle™ 293 Expression Medium is animal origin–free, protein-free and chemically defined, allowing for easier purification of your protein of interest. Gibco® chemically defined media contain no proteins, hydrolysates, or components of unknown composition.Easy use as part of the FreeStyle™ 293 Expression System and FreeStyle™ MAX Expression System for protein productionThe FreeStyle™ 293 Expression System is a complete, suspension cell culture system for generating large amounts of mammalian recombinant protein (see figure). The FreeStyle™ 293 Expression System combines Gibco® FreeStyle™ 293 Expression Medium with 293fectin™ reagent, a cationic-lipid formulation designed to transfect suspension FreeStyle™ 293-F cells at high efficiency. The FreeStyle™ MAX Expression System is a breakthrough technology for rapid and high-yield mammalian protein production. The FreeStyle™ MAX Expression System combines Gibco® FreeStyle™ Media, FreeStyle™ MAX Reagent, and either FreeStyle™ CHO-S cells or FreeStyle™ 293-F cells.Scalability in spinner flasks and bioreactorsProtein production using Gibco® FreeStyle™ 293 Expression Medium can be scaled up in spinner flasks or bioreactors. The appropriate spinner or impeller speed and seeding density should be optimized for each system. Depending on the impeller design and speed, it may be necessary to supplement Gibco® FreeStyle™ 293 Expression Medium with additional Pluronic® F-68 to avoid sheer stress in the culture.Universal bag packagingGibco® universal bags offer flexible packaging that allows you to integrate a single-use, disposable large volume media container with a wide variety of automated cell culture instruments, disposable bioreactor systems, and sterile connection processes, minimizing the need for costly customization.Product useCustomers using Gibco® FreeStyle™ 293 Expression Medium in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from Life Technologies to reference our Type II Drug Master File (DMF).cGMP manufacturing and quality systemGibco® FreeStyle™ 293 Expression Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. 规格

Form:	Liquid
Volume:	20 L
Quantity:	1 universal bag(s)
Cell Line:	FreeStyle™ 293-F Cells
Glutamine:	GlutaMAX™-I
Sterility:	Sterile
Amino Acids:	Non-Essential Amino Acids
Serum Level:	Serum-Free
Concentrated:	1 X
Culture Type:	Suspension Cell Culture
Product Size:	20 L
Classification:	Animal Origin-Free,Protein-Free,Serum-Free,Chemically Defined
Endotoxin Level:	Low
Concentration Ratio:	1 :1
Culture Environment:	CO2
Included Antibiotics:	No Antibiotics
Phenol Red Indicator:	No Phenol Red
Purity or Quality Grade:	Cell Culture Grade
Sodium Pyruvate Additive:	Sodium Pyruvate
Sodium Bicarbonate Buffer:	Sodium Bicarbonate
Shipping Condition:	Room Temperature

内容及储存 Storage conditions: 2-8°C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture