货号：12337024

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：1000 RXNS

目录价：￥18494.00

市场价格：￥16644.60

会员价格：￥16644.60

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 AccuPrime™ GC-Rich DNA Polymerase is designed to provide high-yield, high-specificity amplification of difficult-to-amplify templates, such as those with >65% GC content. The kit offers a choice of buffers for amplifying genomic DNA targets (Buffer A) or non-GC-rich cDNA, plasmid, and lambda-based targets (Buffer B). Benefits of using AccuPrime™ GC-Rich DNA Polymerase:• Yield—high yields for targets up to 5 kb in length• Specificity—AccuPrime™ accessory proteins for improved PCR specificity• Sensitivity—sensitivity down to 5 ng of template DNARobust and specific amplificationsThe AccuPrime™ buffers contain thermostable proteins that enhance primer-template hybridization during PCR, increasing the specificity of the reaction. The polymerase itself, from the archaebacterium Pyrolobus fumarius, has a five-fold better processivity than Taq DNA polymerase, and remains active even after 4 hours at 95°C.Unit definitionOne unit of AccuPrime™ GC-Rich DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of dNTPs into acid-insoluble material in 30 min at 74°C. 规格

Polymerase:	AccuPrime™ GC-Rich DNA Polymerase
Hot Start:	Built-In Hot Start
Fidelity (vs. Taq):	2 X
GC-Rich PCR Performance:	High
Reaction Format:	Separate Components
Product Overhang:	Blunt
Reaction Speed:	Standard
Final Product Size(s):	5 kb or less
Product Size:	1,000 reactions
Exonuclease Activity:	3' - 5'
Shipping Condition:	Approved for shipment on Wet or Dry Ice

内容及储存 AccuPrime™ GC-Rich DNA Polymerase is supplied with two 5X reaction buffers and a separate vial of 50 mM MgSO₄ . Both buffers contain dNTPs, MgSO₄ , and AccuPrime™ accessory proteins. Reaction buffer A is for genomic targets. Reaction buffer B is for use with cDNA, plasmid, or lambda targets. Store at -20°C. Guaranteed stable for 6 months when properly stored.