货号：12183016

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：50 REACTIONS

目录价：￥5320.00

市场价格：￥4788.00

会员价格：￥4788.00

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 The PureLink® RNA Micro Scale Kit provides rapid purification and concentration of total RNA from a variety of samples (e.g., LCM tissues, fine needle aspirates, and FACS-sorted cells). RNA purification using the PureLink® RNA Micro Scale Kit is:• Effective—expect excellent RNA recovery (up to 100 µg total RNA) from small samples• Convenient and safe—the extraction procedure uses no phenol/chloroform, CsCl gradients, or precipitation with either LiCl or ethanol• Fast—obtain high-quality total RNA, typically in just 15 minutes• Reliable—obtain ready-to-use RNA suitable for any downstream applicationA simple and reliable technology for everyday RNA purificationPureLink® technology combines guanidine-isothiocyanate lysis with the speed, purity, and ease-of-use of silica-membrane purification. The safe and easy procedure can be completed in typically less than 15 minutes with >85% recovery from the first elution. The kit can be used for clean-up of purified RNA from enzymatic reactions, desalting of RNA samples after phenol extraction or ethanol precipitation, and concentration of RNA. Purify RNA from up to 10 mg of tissue or 10⁵ cells. Clean up and concentrate RNA amounts ranging from pg to 100 µg in volumes as small as 10 µL. Purified RNA is ready for use in downstream applications such as RT-PCR, qRT-PCR, northern blots, cDNA library construction, and nuclease protection assays.A simple and rapid procedureHomogenize your sample in lysis buffer, add 70% ethanol, and mix. Deposit the resulting solution into a PureLink® Micro kit column with collection tube, and centrifuge for one minute. Nucleic acids bind to the PureLink® silica membrane in the column, and after subsequent washes and on-column digestion with DNase, purified RNA can be eluted with RNase-free water. 规格

Sample Type (General):	Liquid Samples (e.g. Serum),Bacteria,Cells,Plant Samples,Tissue,Yeast
High Throughput Compatibility:	Not High Throughput-Compatible (Manual)
Downstream Application:	RT-PCR (Endpoint),cDNA Library Construction,Nuclease Protection Assays,Nucleic Acid Labeling,Real-Time PCR,Northern Blotting
Scale:	Micro
Format:	Spin Column
System:	PureLink™
Product Size:	50 preps
Final Product:	Total RNA
Yield (up to):	100 µg
Purification Time:	15 min
Elution Volume - max:	10 µl
Elution Volume - min:	5 µl
Isolation Technology:	Silica Spin Column
Sample Type (Specific):	Bacteria,Mammalian Cells,Laser Capture Microdissection (LCM),Yeast,Tissue (Mammalian),Plant,Fine Needle Aspirates (FNA)
Purity or Quality Grade:	Molecular Biology Grade
Starting Material (Amount):	Up to 5 x 10^5 cells,Less than 10 mgs tissue
Shipping Condition:	Room Temperature

内容及储存 Box 1 (PureLink™ RNA Micro Kit): • Lysis Buffer (125 ml) • Wash Buffer I (50 ml) • Wash Buffer II (15 ml) • RNase-Free Water (15.5 ml) • PureLink™ Micro Kit Columns with collection tubes (50 each) • Collection Tubes (50 each) • Recovery Tubes (50 each) Box 2 (PureLink™ DNase/Carrier RNA) • Lyophilized PureLink™ DNase (1500 Units) • PureLink™ On-Column DNase 2X Buffer (1 ml) • RNase-Free Water (1 ml) • Lyophilized PureLink™ Carrier RNA (300 µg) Store Box 2 at 4°C. Store remaining components at room temperature.