货号:1178001K
品牌:ThermoFisher /Invitrogen/ Gibco/ ABI
规格:1000 X 50UL REACTIONS
目录价:¥59119.00
市场价格:¥53207.10
会员价格:¥53207.10
金山科研平台,产品价格货期咨询微信:jinshanbio
描述
EXPRESS One-Step SYBR® GreenER™ SuperMix Kits incorporate proven Invitrogen enzymes now specifically tailored to deliver superior results on high-throughput, fast-cycling instruments for your probe-based qPCR experiments. These kits provide:• Fast-activating, antibody-mediated Platinum® Taq DNA Polymerase in association with the brighter and less PCR-inhibitory SYBR® GreenER™ dye - complete, rapid activation provides the best sensitivity and reproducibility (Figure 1)• The One-Step SuperScript™III Reverse Transcriptase module has a unique formulation to ensure better yield and less qPCR inhibition specifically for one-step qRT-PCR• Flexible format with ROX premixed or separate - designed to work with default instrument protocols on a wide range of high-throughput fast instruments (e.g., AB 7500 Fast, AB 7900 HT Fast, AB StepOne, Roche LightCycler® 480, Corbett Rotor-Gene™) (Figure 1)• Peace of mind with the only commercially available one-step qRT-PCR kits containing heat-labile UDG - significantly reduces carryover contamination risk thanks to its excellent activity from room temperature to approximately 40·C. Due to the thermostability of SuperScript® III Reverse Transcriptase, the cDNA synthesis step is run at 50·C, at which temperature the heat-labile UDG is totally and irreversibly inactivated (Figure 2).Easy to test and switchEXPRESS kits work with manufacturer’s default protocols on fast instruments and come in a convenient and stable SuperMix format for ultimate convenience. Additionally, kits are offered with either premixed ROX or a separate tube of ROX to fit your instrument needs. This "Universal" kit contains a separate tube of ROX.
规格
PCR Method: |
1-Step qRT-PCR |
Detection Method: |
SYBR |
Reaction Speed: |
Standard,Fast |
Platform: |
Cepheid SmartCycler,MJ Opticon,StepOnePlus™, Standard Mode,BioRad CFX96™,StepOnePlus™, Fast Mode,Corbett RotorGene,7500 Fast System,Stratagene Mx4000,Stratagene Mx3005P,Stratagene Mx3000P,MJ Chromo4,7500 System,BioRad MyiQ,BioRad iCycler iQ |
Sample Type (General): |
RNA |
Product Size: |
2,500 reactions |
内容及储存
EXPRESS One-Step SYBR® GreenER™ SuperMix Kits are composed of 2 modules: EXPRESS SYBR® GreenER™ RT module (containing SuperScript® III Reverse Transcriptase and RNAseOUT™) and EXPRESS SYBR® GreenER™ qPCR SuperMix with ROX in a separate tube. Store at -20°C upon receipt. Once thawed, the EXPRESS SYBR® GreenER™ qPCR SuperMix can be stored for up to 1 month at +4·C. Product is guaranteed to be stable for 6 months when properly stored.
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