货号：11755500

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：500 RXN

目录价：￥64178.00

市场价格：￥57760.20

会员价格：￥57760.20

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 SuperScript® VILO™ Master Mix is a robust reverse transcriptase premix containing everything you need for reverse transcription (RT) in one tube. SuperScript® VILO™ (Variable Input, Linear Output) Master Mix pairs our tried and tested SuperScript® III Reverse Transcriptase with enhanced buffer and RNaseOUT™ Recombinant Ribonuclease Inhibitor to enable reliable first-strand synthesis and higher cDNA yields from a wide range of input RNA concentrations. This versatility helps deliver unprecedented linearity across the broadest range of input material and increased dynamic range for your quantitative RT-PCR (qRT-PCR) assays. Features of the SuperScript® VILO™ Master Mix:• Sensitive—linearity from 1 pg to 2.5 µg in a 20 µL reaction• Convenient—single-tube format, just add your RNA and go• Flexible—suitable for qPCR detection methods such as SYBR® Green and TaqMan® assays• Powerful—high yields of cDNA perfect for archivingConsistent results from a variety of input RNA concentrationsThe SuperScript® VILO™ Master Mix provides the high-temperature capability of SuperScript® III Reverse Transcriptase in an optimized format. The SuperScript® VILO™ Master Mix can generate first-strand cDNA for use in real-time quantitative RT-PCR (qRT-PCR) with very low and very high amounts of input RNA (up to 2.5 µg total RNA in a 20 µL reaction).Reliable, RNA-protected, reverse transcripition from total RNABecause SuperScript® III RT is not significantly inhibited by ribosomal and transfer RNA, it can be used to synthesize cDNA from total RNA. The SuperScript® VILO™ Master Mix also includes RNaseOUT™ Recombinant Ribonuclease Inhibitor to safeguard against the degradation of target RNA due to ribonuclease contamination. Robust SuperScript® III RT can be used to synthesize cDNA at a temperature range of 42–55°C.Broad linearity for less template dilutionsObtain the same relative representation in your cDNA qPCR template, regardless of gene abundance. During normalization, get both your gene of interest and the reference gene in a linear phase for cDNA synthesis even if their abundances differ in the starting material. 规格

Optimal Reaction Temperature:	42° C
Final Product Size(s):	10 kb or less
Reverse Transcriptase:	SuperScript® III
Ribonuclease H Activity:	Reduced
Form:	Liquid
Template:	ssRNA
Product Size:	500 reactions
Enzyme Function:	Reverse Transcriptase
Shipping Condition:	Dry Ice
Number of Reactions:	500 Reactions

内容及储存 SuperScript® VILO™ Master Mix (200 µL) includes: SuperScript® III RT, RNaseOUT™ , Recombinant Ribonuclease Inhibitor, a proprietary helper protein, random primers, MgCl₂, and dNTPs. Store at -20°C upon receipt.