货号：11754100

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：100X50 UL RXNS

目录价：￥6170.00

市场价格：￥5553.00

会员价格：￥5553.00

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 The CellsDirect™ One-Step qRT-PCR Kit delivers highly sensitive and specific real-time qPCR results directly from cells, without the need for an RNA purification step.Features of the CellsDirect™ One-Step qRT-PCR Kit include:• Efficiency—Offers significant time and cost savings for increased throughput• Convenience—Enzyme mix includes both SuperScript® III Reverse Transcriptase and Platinum® Taq DNA Polymerase• Sensitivity—No separate RNA isolation step increases sensitivity by minimizing sample loss• Broad Detection Range—Detect targets from samples ranging from a single cell to 10,000 cells• Versatility—Compatible with a wide variety of cell lines and samples collected by Laser Capture Microdissection (LCM)Streamline Your Workflow In traditional real-time quantitative RT-PCR (qRT-PCR), you first isolate RNA from cells in a time-consuming procedure that can lead to loss of material. Using the CellsDirect™ One-Step qRT-PCR Kit, you lyse the cells and add the complete lysate directly to a one-step qRT-PCR reaction with minimal handling and sample loss.Ideal for Your qRT-PCR Application CellsDirect™ technology is validated for gene expression studies involving limited samples, and is suitable for validation of RNAi knockdowns. CellsDirect™ One-Step qRT-PCR Kits are especially valuable for high-throughput applications by eliminating costly, time-consuming RNA purification steps (Figure 1). The CellsDirect™ One-Step qRT-PCR Kit may also be used to detect genomic DNA targets. CellsDirect™ One-Step qRT-PCR Kits may be used in conjunction with hydrolysis probes (e.g. TaqMan®) or LUX™ fluorogenic primers.Note: This kit includes 2X Reaction Mix containing ROX™ Reference Dye. An equivalent kit (11753-100), containing a separate tube of ROX™ Reference Dye, is also available. 规格

PCR Method:	1-Step qRT-PCR
Detection Method:	Primer-Probe Detection
Reaction Speed:	Standard,Fast
Platform:	7300 System,7900HT System,Eppendorf® MasterCycler® RealPlex,7900HT Fast System,7700 System,7000 System
Sample Type (General):	Cells
Product Size:	100 reactions

内容及储存 • 10 ml Resuspension Buffer • 1 ml Lysis Enhancer • 500 µl DNase I, Amplification Grade (1 U/µl) • 160 µl 10X DNase I Buffer • 400 µl 25 mM EDTA • 100 µl SuperScript® III RT/Platinum® Taq Mix • 2 × 1.25 ml 2X Reaction Mix with ROX™ • 1 ml 50 mM MgSO₄ • 2 ml DEPC-treated water • 10 µl HeLa Total RNA (10 ng/µl) The kit is shipped on dry ice and should be stored at -20°C