货号:11448032
品牌:ThermoFisher /Invitrogen/ Gibco/ ABI
规格:2500 UN #Y02028 #95514
目录价:¥30817.00
市场价格:¥27735.30
会员价格:¥27735.30
金山科研平台,产品价格货期咨询微信:jinshanbio
描述
Platinum® GenoType Tsp DNA Polymerase is designed and qualified specifically for amplification of dinucleotide repeat markers in PCR-based genotyping applications. The enzyme is pre-complexed with a thermolabile inhibitor containing monoclonal antibodies to Tsp DNA polymerase, providing an automatic "hot-start" method that improves PCR specificity and allows for room temperature reaction assembly. Platinum® GenoType Tsp DNA Polymerase:• Exhibits minimal non-templated nucleotide addition to PCR products• Lacks both 5´ and 3´ exonuclease activity• Amplifies fragments up to 500 bpApplicationAmplification of dinucleotide repeat loci in applications in which elimination of non-templated nucleotide addition is desirable.Unit definitionOne unit of Platinum® GenoType Tsp DNA Polymerase has been functionally determined to be equivalent to one unit of Taq DNA Polymerase in amplification of dinucleotide repeats using standard Taq DNA polymerase reaction conditions. One Tsp DNA polymerase unit approximates 2.5 activity units. An activity unit incorporates 10 nmoles of deoxyribonucleotide into acid-precipitable material in 30 min at 74°C under optimized reaction conditions.
规格
Polymerase: |
Tsp Genotyping Polymerase |
Hot Start: |
Built-In Hot Start |
Fidelity (vs. Taq): |
1 X |
GC-Rich PCR Performance: |
Low |
Reaction Format: |
Separate Components |
Product Overhang: |
Blunt |
Reaction Speed: |
Standard |
Final Product Size(s): |
500 bp or less |
Product Size: |
2500 units |
Exonuclease Activity: |
None |
Shipping Condition: |
Approved for shipment on Wet or Dry Ice |
内容及储存
Contents:
• 1 tube of 500 µL (5
Tsp U/µL)
• 10X PCR Buffer (200 mM Tris-HCl pH 8.4, 500 mM KCl)
• 1 vial of 50 mM MgCl
2
Store at -20°C. Guaranteed stable for 6 months when properly stored.
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