货号：11429D

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：5 ML

目录价：￥12651.00

市场价格：￥11385.90

会员价格：￥11385.90

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 Use this kit to enrich viable mouse dendritic cells (DC's) from spleen/lymph node cells by negative isolation. The kit depletes T cells, mIgM+ B cells, NK cells, erythrocytes and most granulocytes. Highly enriched mouse DC's are bead and antibody-free, and can be used in any functional assay. The cells can be further sorted to obtain high purity of specific DC sub-populations.Obtain high recovery (80-100%) of viable Lin-CD11c+ DC's of all sup-populations, including DC's positive for CD4, CD8α, B220 and CD19.• Substantial reduction in sorting time and volume compared to sorting only, thus saving money in addition.• Pool cells/tissue for easy sample scale -upMethodology:An antibody mix towards the non-dendritic cells is added to the sample and allowed to bind to the cells. Dynabeads® are added and will bind to the antibody-labeled cells during a short incubation. The bead-bound cells are quickly separated on a magnet and discarded. The remaining untouched mouse DC's can be directly sorted into the sup-population of choice or used in any other application.Starting samples:Spleen or lymph node cells. 规格

Capacity:	Processes ~1x10^9 cells total
Cell Type:	Dendritic Cells
Reactivity:	Mouse
Product Size:	5 mL
Target Species:	Mouse
Isolation Method:	Negative Isolation
Output Viability:	>95%
Sample Type (Specific):	Spleen,Lymph Node
Starting Material (Cell #):	1x10^7 cells per isolation
Shipping Condition:	Room Temperature

内容及储存 The Kit Contains: 5 ml Depletion MyOne™ Streptavidin Dynabeads® and 1 ml Antibody Mix containing an optimized mixture of biotinylated monoclonal antibodies towards mouse CD2, CD3ε, CD49b, mIgM and Ter-119.Store at 2-8°C