货号:11361D
品牌:ThermoFisher /Invitrogen/ Gibco/ ABI
规格:3 ML 2X10E9
目录价:¥14997.00
市场价格:¥13497.30
会员价格:¥13497.30
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描述
This gentle and easy-to-use kit allows you to isolate flow-compatible human CD4+ T-cells from PBMC or directly from whole blood. Immediately after positive isolation, the beads are released and removed from your cell sample. In this way, you avoid exposing your cells to potentially cytotoxic or immunogenic foreign substances like iron oxides or dextrans, a potential problem when working with biodegradable particles. In addition, your cells will not be exposed to the stress of being passed through a column.You can expect a purity level of >95% and up to 75% recovery of human CD4+ cells.Advantages:• No risk of your results being affected by the isolation method• Gentle tube-based method - no columns needed• High purity and recovery• Bead-free cells - compatible with flow cytometry analysis• Viable, bead-free CD4+ T-cells for direct use in any cell-based assay.The isolated cells have the presumed profile of memory and naive CD4+ T cells, and show a high proliferating and functional capacity. This kit avoids contamination of your isolated CD4+ cells with CD14+ monocytes. You will save time and money, and get increased confidence in your results.Starting sample: MNC or whole bloodCapacity:The kit will process 2 × 109 mononuclear cells (MNC) or 80 ml whole blood.
规格
Capacity: |
Processes ~2x10^9 cells total |
Cell Type: |
T Cells (CD4) |
Product Size: |
3 mL |
Target Species: |
Human |
Isolation Method: |
Positive Isolation |
Output Viability: |
>95% |
Sample Type (Specific): |
Blood,PBMC |
Starting Material (Cell #): |
5x10^7 PBMCs or 2 ml whole blood per isolation |
Shipping Condition: |
Room Temperature |
内容及储存
The Kit Contains 1 ml Antibody, 3 ml FlowComp™ Dynabeads® and 40 ml FlowComp™ Release Buffer.
Store at: 2-8°C
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