货号:11352D
品牌:ThermoFisher /Invitrogen/ Gibco/ ABI
规格:2 ML, 2X10E8
目录价:¥3461.00
市场价格:¥3114.90
会员价格:¥3114.90
金山科研平台,产品价格货期咨询微信:jinshanbio
描述
Use this kit to isolate pure and viable untouched CD4+ T-cells from PBMC by negative isolation. The kit depletes CD8+ T cells, B cells, NK cells, monocytes, platelets, dendritic cells, granulocytes and erythrocytes. The negatively isolated human CD4+ T-cells are left in the sample and have not been in contact with the Dynabeads. The bead and antibody-free CD4+ T-cells are truly untouched, and in perfect shape for any functional assay and application.• High purity, recovery and viability of human untouched CD4+ T cells• Easy to use and to scale up• No columns requiredMethodology: An antibody mix towards the non-CD4 cells is added to the sample and allowed to bind to the cells. Dynabeads® are added and will bind to the antibody-labeled cells during a short incubation. The bead-bound cells are quickly separated on a magnet and discarded. The remaining negatively isolated and untouched human CD4+ T-cells can be directly analyzed in a flow cytometer and used in any application.Note: A negative isolation strategy isolates pure and truly untouched cells. When maximum cell purity is critical, a positive cell isolation strategy is recommendedStarting Sample:PBMC
规格
Capacity: |
Processes 2x10^8 cells |
Cell Type: |
T Cells (CD4) |
Product Size: |
2 mL |
Final Product: |
Cells |
Target Species: |
Human |
Isolation Method: |
Negative Isolation |
Sample Type (Specific): |
MNC |
Shipping Condition: |
Room Temperature |
内容及储存
The Kit Contains: 2 ml Depletion Dynabeads® and 0.4 ml Antibody Mix with monoclonal antibodies
towards human CD8, CD14, CD16 (a and b), CD19, CD36, CD56, CD123 and CD235a
(Glycophorin A). Store at 2-8°C
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