货号:11306081
品牌:ThermoFisher /Invitrogen/ Gibco/ ABI
规格:5000 REACTIONS
目录价:¥63464.10
市场价格:¥57117.69
会员价格:¥57117.69
金山科研平台,产品价格货期咨询微信:jinshanbio
描述
Platinum® PCR SuperMix is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification. All you have to do is add template and primers, reducing set-up time by half (see figure). Platinum® PCR SuperMix:• Allows for room temperature reaction assembly• Amplifies fragments up to 5 kb• Produces products with 3'-A ends• Improves PCR specificityUsing Platinum® PCR SuperMixPlatinum® PCR SuperMix is for high-specificity DNA amplification. High specificity is provided by a mixture of recombinant Taq DNA Polymerase and Platinum® monoclonal antibodies to Taq DNA Polymerase. Automatic "hot-start" improves the specificity of PCR. Taq DNA Polymerase is activated during the initial denaturation step.
规格
Hot Start: |
Built-In Hot Start |
Fidelity (vs. Taq): |
1 X |
GC-Rich PCR Performance: |
Low |
Product Overhang: |
3'-A |
Final Product Size(s): |
5 kb or less |
Product Size: |
5,000 reactions |
Exonuclease Activity: |
5' - 3' |
Shipping Condition: |
Approved for shipment on Wet or Dry Ice |
内容及储存
Platinum® PCR SuperMix is supplied at 1.1X concentration to allow approximately 10% of the final reaction volume to be used for the addition of primer and template solutions. Reagents sufficient for 5,000 amplification reactions of 50 ?l each are provided.
Platinum® PCR SuperMix Components:
22 U/ml complexed recombinant Taq DNA polymerase with Platinum® Taq Antibody, 22 mM Tris-HCl (pH 8.4), 55 mM KCl, 1.65 mM MgCl2, 220 ?M dGTP, 220 ?M dATP, 220 ?M dTTP, 220 ?M dCTP, and stabilizers.
5,000 rxns: 4 × 56.25 ml
Sore at -20°C in a non-frost-free freezer.
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