货号：11161D

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：0.4 ML

目录价：￥2049.00

市场价格：￥1844.10

会员价格：￥1844.10

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 Dynabeads® Human T-Activator CD3/CD28 are for the activation and expansion of human T cells. Advantages of Dynabeads® Human T-Activator CD3/CD28: • Activation of T cells without the need for feeder cells • Activated cells that retain in vivo-like function • Maximum reproducibility without contamination by soluble antibodies or mitogens • From 100- to 1,000-fold T cell expansionAbout Dynabeads® Human T-Activator CD3/CD28 Dynabeads® Human T-Activator CD3/CD28 offer a simple method for activation and expansion of T cells that does not require feeder cells (antigen-presenting cells) or antigen. The uniform 4.5 µm diameter, inert, superparamagnetic beads are similar in size to antigen-presenting cells, and are covalently coupled to anti-CD3 and anti-CD28 antibodies. These two antibodies provide primary and co-stimulatory signals, optimized for efficient T cell activation and expansion. Expansion of the T cell population can be stimulated using recombinant IL-2, and after activation or expansion, the magnetic beads can be easily removed using a DynaMag™ magnet. For expansion of antigen-specific T cells from T cell clones or T cell lines, we recommend using Dynabeads® Human T-Activator CD3/CD28/CD137.Starting samples Start with mononuclear cells (MNCs)/peripheral blood mononuclear cells (PBMCs) from either whole blood or buffy coat, or T cell subsets including CD3+ T cells, CD4+ or CD8+ T cells, or regulatory T cells.Downstream applications The activated T cells can be analyzed shortly after activation (for transfection/transduction or to study TCR signaling, proteomics, gene expression, etc.). T cells can be left in culture to differentiate into T helper cell subsets or expansion of polyclonal/antigen-specific T cells.Learn more about Dynabeads® products • Find Dynabeads® products for a whole range of applications. • Find magnets for Dynabeads® separations. 规格

Form:	Bead
Host:	Mouse
Ligand:	Antibody
Cell Type:	T Cells (Whole Population)
Clonality:	Monoclonal
Reactivity:	Human
Product Size:	0.4 mL
Final Product:	Activated, expanded T cells
Target Species:	Human
Isolation Method:	Dynabeads Untouched or Dynabeads FlowComp
Number of Reactions:	200 Tests
Surface Functionality:	Epoxy
High Throughput Compatibility:	Not High Throughput-Compatible (Manual)
Shipping Condition:	Room Temperature

内容及储存 The product contains 0.4 ml Dynabeads Human T-Activator CD3/CD28 in phosphate buffered saline (PBS), pH 7.4, containing 0.1% human serum albumin (HSA). Store at 2–8°C 4 × 10⁷ beads/ml suspension.