货号：11146057

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：COMBO

目录价：￥3236.00

市场价格：￥2912.40

会员价格：￥2912.40

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 ThermoScript™ Reverse Transcriptase (RT) is an avian RT with reduced RNase H activity designed for RT-PCR of the most highly structured, difficult RNA templates. The ThermoScript™ RT-PCR Systems are designed to copy RNA with secondary structure and to improve specificity of priming. In conjunction with PCR, the system can be used to detect the presence of rare messages or to quantitate the amount of specific mRNA from small numbers of cells. The systems contain ThermoScript™ RT, a cloned avian reverse transcriptase that has been engineered to have reduced RNase H activity and to have high thermal stability (up to 70°C). Each system is optimized for generating first-strand cDNA from total or poly(A)+ RNA (1) (Figure 1). ThermoScript™ RT-PCR Systems:• Are optimized for RT-PCR• Can be used with highly structured RNA or gene-specific primers• Reduce RNase H activity for greater first-strand cDNA yield• Amplify first-strand cDNA products directly without organic extractions or precipitations• Include oligo(dT) and random hexamers to prime cDNA synthesis for flexible use• Offer a choice of Platinum® Taq DNA Polymerase for higher specificity (2) (Figure 2) when amplifying templates up to 3 kb, or Platinum® Taq DNA Polymerase High Fidelity for amplifying templates up to 12 kb (Figure 3) 规格

PCR Method:	2-Step RT-PCR
Reverse Transcriptase:	ThermoScript™
Polymerase:	Platinum® Taq DNA Polymerase
Hot Start:	Built-In Hot Start
Sample Type (General):	RNA
Product Size:	25 reactions
Detection Method:	Gel Electrophoresis
Final Product Size(s):	3 kb or less
Primer-Probe Compatibility:	Unlabeled Oligos

内容及储存 • 24 µl Platinum® Taq DNA Polymerase • 1.25 ml 10X PCR buffer (no magnesium) • 1 ml 50 mM Magnesium Chloride • 1.3 ml KB Extender • 25 µl ThermoScript™ RT (15 U/µl) • 500 µl 5X cDNA Synthesis Buffer • 250 µl 0.1 M DTT • 100 µl 10 mM dNTP Mix • 25 µl RNaseOUT™ (40 U/µl) • 25 µl Oligo (dT)₂₀ (50 µM) • 50 µl Random Hexamers (50 ng/µl) • 1.25 ml DEPC-Treated Water • 50 µl E. coli RNase H (2 U/µl) Store at -20°C (stability can be extended by storage at -70°C).