货号：10605HNAE25

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：25 UG

目录价：￥692.00

市场价格：￥622.80

会员价格：￥622.80

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 Manufactured by Sino Biological (China); Distributed by Life Technologies. Epidermal growth factor (EGF) recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications.This recombinant protein was expressed from a DNA sequence encoding the mature form of human EGF (NP_001954.2) (Asn 971-Arg 1023) retaining the initial Met at the N-terminus.N-terminal Sequence Analysis: Met 1Activity: Measured in a cell proliferation assay using BALB/c 3T3 mouse embryonic fibroblasts (Rubin, J.S. et al. 1991, Proc. Natl. Acad. Sci. USA 88:415.) . The EC₅₀ for this effect is typically 20-100 pg/mL.Formulation: Lyophilized in 140 mM NaCl, 2.7 mM KCl, 10 mM Na₂HPO₄, 1.8 mM KH₂PO₄, pH 7.4, 5% mannitol, 5% trehalose, 0.02% Tween®-80.Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.Expiration Date: Expires one year from date of receipt when stored as instructed.BackgroundHuman epidermal growth factor (EGF) exerts actions on a wide variety of biological processes, and plays an important role in the regulation of cell growth, proliferation, and differentiation mediated by its high-affinity receptor EGFR on the cell surface. It induces differentiation of specific cells, promotes angiogenesis, and inhibits gastric acid secretion in vivo, while serves as a potent mitogenic actor for a variety of cultured cells of both ectodermal and mesodermal origin. Human EGF was originally isolated from urine based on its inhibitory effect on gastric secretion and named urogastrone accordingly. EGF is the founding member of the EGF-family of proteins which are characterized by the presence of at least one EGF structural unit (a conserved 6 cysteine motif that forms three disulfide bonds). The EGF precursor is believed to exist as a membrane-bound molecule containing 9 EGF units, and is proteolyticallycleaved to generate the 53-amino acid peptide hormone.You may also be interested in the following EGF products: KinasesPrimary AntibodiesBioconjugates 规格

Form:	Lyophilized
Purity:	≥ 90% by SDS-PAGE
Species:	Human
Expression:	E. coli
Gene Symbol:	EGF
Gene Aliases:	EGF, HOMG4, URG
Product Size:	5 x 5 µg
Protein Form:	Full Length,Recombinant
Protein Family:	Growth Factors & Receptors
Protein Subtype:	EGF (Epidermal Growth Factor)
Molecular Weight:	6.3 kDa
Research Category:	Angiogenesis,Cell Cycle & Proliferation
Protein Tag or Fusion:	Untagged
Validated Application:	Assay Standard

内容及储存 Includes 5 vial of lyophilized protein. Store at -20 °C.