货号：10533065

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：500 UN.

目录价：￥1397.00

市场价格：￥1257.30

会员价格：￥1257.30

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 Terminal Deoxynucleotidyl Transferase, Recombinant (rTdT) is a DNA polymerase that catalyzes the addition of deoxynucleotides to the 3fi hydroxyl terminus of DNA. A TdT Technical Bulletin is available.Applications: Homopolymer tailing of vector and insert for cloning. Labeling oligonucleotides with biotin (1,2), ³²P- or ³⁵S-label (3), or in apoptosis (TUNEL) (4,5).Source: Purified from a baculovirus clone of calf thymus TdT.Performance and Quality Testing: Endonuclease, 3´ and 5´ exodeoxyribonuclease, and levels of incorporation tested.Unit Definition: One unit incorporates 1 nmol dATP into acid-precipitable material in 1 h at 37°C, using d(pA)₅₀ as a primer.Hazard Warning: Toxic; potassium cacodylate contained in reaction buffer. Also contains cobalt chloride, a highly toxic chemical.See MSDS.Unit Reaction Conditions: 0.2 M potassium cacodylate (pH 7.2), 10 mM MgO₄ C₄ H₆ , 1 mM 2-mercaptoethanol, 0.5 mg/ml BSA,100 flM d(pA)₅₀, 1 mM [³H]dATP, and enzyme in 0.15 ml for 1 h at 37°C. 规格

Enzyme:	Terminal Deoxynucleotidyl Transferase
Product Size:	500 units
Shipping Condition:	Approved for shipment on Wet or Dry Ice

内容及储存 Terminal Deoxynucleotidyl Transferase, Recombinant (rTdT) is supplied with vial of 5X buffer [500 mM potassium cacodylate (pH 7.2), 10 mM CoCl₂ , 1 mM DTT]. Store at -20°C.