货号：10009D

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：50 ML

目录价：￥67926.00

市场价格：￥61133.40

会员价格：￥61133.40

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 Dynabeads® Protein G are uniform, 2.8 µm superparamagnetic beads with recombinant Protein G (approximately 17 kDa) covalently coupled to the surface. Dynabeads® Protein G provide a superior alternative to Sepharose® or agarose slurry for immunoprecipitation (IP):• Simple, short ~30 minute procedure• Significantly reduces background caused by nonspecific binding• No columns, centrifugations, or time-consuming pre-treatment of your samples • Gentle separation technology preserves native protein conformations and large-protein complexesMagnetic bead-based separation is rapid and easy to performAntibody is added to the Dynabeads® Protein G. During a short incubation, the antibody binds to the Dynabeads® via their Fc-region. The tube is then placed on a magnet, where the beads migrate to the side of the tube facing the magnet. The supernatant is then removed by aspiration. The bead-bound antibody may now be used for a variety of downstream applications including immunoprecipitation, co-IP, small-scale IgG purification and antibody labeling, chromatin immunoprecipitation (ChIP), RNA IP (RIP), and protein isolation. Bound material is easily collected on a DynaMag™ magnet, utilizing the unique magnetic properties of the Dynabeads®. The recombinant protein G on the beads contains no albumin binding sites, thus albumin is not co-purified during the procedure.Gentle separation causes minimal physical stress to proteinsThe magnetic separation technology utilized by Dynabeads® Protein G is rapid and gentle, causing minimal physical stress to your target proteins. This permits the isolation and concentration of labile composites that might otherwise dissociate or be damaged by proteases during long incubation times. Native protein conformation and large protein complexes are preserved.Binding strength and capacityDynabeads® Protein G allows for isolation of most mammalian immunoglobulins (Ig). (The Binding strengths table below gives the binding strengths for different species and subclasses.) The amount of Ig captured depends on the concentration of Ig in the starting sample and on the type and source of the Ig. 100 µL Dynabeads Protein G will isolate approximately 25–30 µg human IgG from a sample containing 20–200 µg IgG/mL. Predominant Fc binding allows optimal Ig orientation Useful for many sample typesUsing Dynabeads® Protein G, antibodies can be purified from saliva, plasma, ascites, serum and tissue culture or hybridoma supernatants.Learn more about Dynabeads®• Dynabeads® Protein G is also available as a "ready-to-go" kit with buffers included.• Find selection guides, data, and references that support the utility of Dynabeads® for immunoprecipitation.• Find magnets for Dynabeads® separations.• Find Dynabeads® products for other applications.br>OEM purchaseTo purchase Dynabeads® Protein A and Protein G on an OEM basis, contact our Out-Licensing and OEM Sales department.*Sepharose® is a trademark of GE Healthcare companies. 规格

Ligand Type:	Protein G
Form:	Beads in Suspension
Shelf Life:	24 months
Product Size:	50 mL
High Throughput Compatibility:	Not High Throughput-Compatible (Manual)
Shipping Condition:	Room Temperature

内容及储存 Contains: 50 mL Dynabeads® Protein G Storage: 2°C to 8°C