货号：558546

品牌：BD Pharmingen

规格：50Tst

目录价：￥3675.00

市场价格：￥2315.25

会员价格：￥2021.25

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p47v-crk (v-Crk) is the product of a transforming gene, v-crk, that was isolated from avian sarcoma viruses. The v-Crk protein is a fusion product of viral Gag protein and a part of cellular Crk that includes SH2 and SH3 domains. v-Crk-induced transformation increases tyrosine phosphorylation of several cellular proteins, including p130Cas (CRK-associated substrate). The p130Cas is tightly associated with v-Crk via the SH2 domain of v-Crk. Tyrosine phosphorylation of p130Cas occurs in conjunction with cellular transformation in cells that express v-Src or v-Crk. This phosphorylation leads to a change in p130Cas localization from the cytoplasm to the cell membrane and, possibly, to the nucleus. Since p130Cas also associates with v-Src, it may be a v-Src substrate. Several phosphorylation sites have been described in p130Cas upon Fibroblast Growth Factor stimulation, and phosphorylated tyrosine (Y249) might function as a binding site for the Crk-adaptor molecule.The J169-757.12.2 monoclonal antibody recognizes the phosphorylated Y249 of human p130Cas. The orthologous phosphorylation sites in mouse and rat p130Cas are Y253 and Y347, respectively.

Catalog Number

558546

Brand

BD Phosflow™

Name

p130^Cas (pY249)

Size

50Tests

RegulatoryStatus

RUO

Clone

J169-757.12.2

Format

Alexa Fluor® 488

Isotype

IgG_2b, κ

W.B. Positive Control

none

Molecular Weight

130 kDa

Immunogen

Phosphorylated Human p130Cas

Reactivity

QC Testing : Human

Applications

Intracellular staining (flow cytometry)

- Routinely Tested