货号：558527

品牌：BD Pharmingen

规格：1EA

目录价：￥4368.00

市场价格：￥2751.84

会员价格：￥2402.40

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Brutonʼs tyrosine kinase (Btk) is a nonreceptor tyrosine kinase whose function is critical for proper B cell development and signaling. It is a member of the Tec family of kinases which includes Tec and Itk. In addition to an N-terminal pleckstrin homology (PH) domain, the Tec proteins contain Src homology domains 2 and 3 (SH2 and SH3) and a stretch of 60-80 amino acids between the PH and SH3 domains termed the Tec homology domain. The activity of Btk is regulated by Src-mediated phosphorylation of the kinase domain at tyrosine 551. This event induces Btk kinase activity and subsequent autophosphorylation at tyrosine 223 in the SH3 domain. Phosphorylated Btk then associates with the cell membrane via the interaction of the PH domain with phosphatidylinositol 3, 4, 5-triphosphate. The PH domain is essential for proper activation and function of Btk. A mutation in the PH domain results in Xid, murine X-linked immunodeficiency, and human X-linked agammaglobulinemia.The 53/BTK monoclonal antibody recognizes human Btk, regardless of phosphorylation status. It does not cross-react with Itk.

Catalog Number

558527

Brand

BD Phosflow™

Name

Btk

Size

50Tests

RegulatoryStatus

RUO

Clone

53/BTK

Format

PE

Isotype

IgG_2a

W.B. Positive Control

EB-1

Immunogen

Human N-Terminal Btk aa. 2-172 Recombinant Protein

Reactivity

QC Testing : Human

Applications

Intracellular staining (flow cytometry)

- Routinely Tested