货号：558518

品牌：BD Pharmingen

规格：50T

目录价：￥4378.00

市场价格：￥2758.14

会员价格：￥2407.90

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Engagement of the T cell receptor (TCR) induces signal transduction pathways that enhance gene transcription and cellular proliferation and differentiation. TCR ligation results in the recruitment and activation of multiple protein tyrosine kinases (PTKs), including lck, fyn, and ZAP70. Adaptor proteins, such as Grb2 and SLP-76, relay the signal to downstream effector molecules. LAT (linker for activation of T cells) is a substrate of the activated ZAP70 and functions to bridge the activated TCR and its associated PTKs with tyrosine kinase substrates. LAT is expressed as 36- and 38-kDa forms that result from post-translational modification, and as a 42-kDa form that results from alternative splicing. LAT is an integral membrane protein that is phosphorylated at five tyrosine sites upon TCR ligation. Following phosphorylation, LAT binds a number of important signaling molecules, including Grb2, Vav, PLCg1, and the p85 subunit of PI3K. Multiple studies have shown that functional LAT is required for T lymphocyte activation and thymocyte development.The I58-1169 monoclonal antibody recognizes the phosphorylated tyrosine 171 (pY171) of LAT, which is one of the phosphotyrosine sites required for binding phosphoinositide 3-kinase, Grb2, and Gads.

Catalog Number

558518

Brand

BD Phosflow™

Name

LAT (pY171)

Size

50Tests

RegulatoryStatus

RUO

Clone

I58-1169

Format

Alexa Fluor® 647

Isotype

IgG₁, κ

W.B. Positive Control

none

Molecular Weight

38 kDa

Immunogen

Phosphorylated Human LAT

Strain Host

BALB/c

Reactivity

QC Testing : Human

Applications

Intracellular staining (flow cytometry)

- Routinely Tested