货号：558356

品牌：BD Pharmingen

规格：100ug

目录价：￥8390.00

市场价格：￥5285.70

会员价格：￥4614.50

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A number of cytoskeletal proteins are tyrosine phosphorylated in Rous sarcoma virus-transformed chick embryo fibroblasts. One of these is the 68-kDa paxillin protein. Paxillin is a cytoskeletal component that localizes to the focal adhesions at the ends of actin stress fibers. It is also present in the focal adhesions of Madin-Darby canine kidney epithelial cells, but is absent from the cell adherens junctions of these cells. Paxillin purified from chicken gizzard migrates as a diffuse band on SDS-PAGE with molecular weight of 65-70 kDa. It binds to the rod domain of vinculin, another focal adhesion protein. Paxillin is a substrate for several tyrosine kinases such as src, FAK, and P210BCR/ABL, and the phosphorylation of paxillin on tyrosine 31 (Y31) is affected by conditions that change cell-cell adhesion. This is consistent with the possibility that paxillin is involved in the regulation of cell morphology. Additionally, because of its SH3-binding domain, paxillin associates tightly with FAK and Crk in an extracellular matrix-independent manner. Although paxillin was initially detected in fibroblasts, its phosphorylation may also be important during neurite extension.The 19/Paxillin (Y31) monoclonal antibody recognizes the phosphorylated Y31 of activated paxillin.

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.Store undiluted at 4° C.

Catalog Number

558356

Brand

BD Pharmingen™

Name

Paxillin (pY31)

Concentration

0.5 mg/ml

Size

0.1mg

RegulatoryStatus

RUO

Clone

19/Paxillin (Y31)

Format

Purified

Isotype

IgG₁, κ

W.B. Positive Control

none

Molecular Weight

68 kDa

Strain Host

BALB/c

Reactivity

QC Testing : Human

Applications

Western Blot

- Routinely Tested