货号:AQ301B
品牌:Merck Millipore
规格:EA
目录价:询价
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Description:
Sheep anti-Rabbit IgG, F(ab')2, biotin-SP conjugate
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Promotional Text:
Special Shipping Offer on Antibodies100% Performance Guaranteed
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Trade Name:
Chemicon (Millipore)
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Product Overview:
Affinity purified sheep anti-rabbit IgG, biotin conjugated, F(ab')2 fragments. Shows minimum cross-reactivity to Human IgG. PURIFICATION: The antibody is isolated from antisera by immunoaffinity chromatography using antigen coupled to Sepharose beads.
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Species Reactivity:
Rabbit
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Application Notes:
The highly specific reagent allows the reliable and sensitive detection of rabbit IgG in various situations where interference of immunoglobulins of other species than rabbit may be expected. The absence of the "sticky" Fc-portion further prevents binding to Fc-receptors and reduces interaction with hydrophobic substances. Thus, the reagent is particularly suitable for use on sections, cells surfaces in double staining techniques and immunoblots.The biotinylated antibody can be used in a number of immunological detection systems (Savage 1986; Wilchek & Baer 1988) such as ELISA, immunohistochemistry or immunocytochemistry, immunoblotting and also for screening on monoclonal antibodies, whereby the use of various streptavidin conjugates allows high flexibilty. RECOMMENDED DILUTIONS: ELISA: 1:5,000 - 1: 10,000 Immunohistochemistry: 1: 250 - 1: 500 Immunoblotting: 1: 500 - 1: 1,000 Optimal working dilutions must be determined by the end user.
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Presentation:
Lyophilized from PBS, pH 7.1 with 15 mg/mL bovine serum albumin (BSA) and 0.1% sodium azide. Reconstitute with 500 μL of sterile distilled water for a concentration of 1 mg/mL.
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Storage Conditions:
Maintain lyophilized material at 2-8°C for up to 12 months. After reconstitution maintain at -20°C in undiluted aliquots for up to six months. Repeated freezing and thawing should be avoided.
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
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ELISA
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Western Blotting
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Brand Family:
Chemicon
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Product Name:
Sheep anti-Rabbit IgG, F(ab')2, biotin-SP conjugate
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Antibody Type:
Polyclonal Antibody
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Qty/Pk:
500 µg
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Format:
Biotin
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Host:
Sheep
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