金山科研平台，产品价格货期咨询微信：jinshanbio Source:E. coli-derived, Contains an N-terminal Ser-Tyr-Tyr and 6-His tag Accession #:AAH33711 Purity:>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain. Predicted Molecular Mass:44 kDa Activity:Recombinant Human His6-Ataxin-3 is a Ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-Ataxin-3 concentration of 1-5 µM. Formulation:Supplied as a solution in HEPES, NaCl, Glycerol, EDTA and DTT.See Certificate of Analysis for details. Molecule Information: Ataxin-3 Aliases: AT3; ATXN3; JOS; MJD1; SCA3 Entrez Gene IDs: 4287 (Human); 110616 (Mouse); 60331 (Rat) Background: Ataxin-3 Ataxin-3, also known as Machado-Joseph Disease (MJD) Protein 1 and Spinocerebellar Ataxia Type 3, is a 364 amino acid (aa), ubiquitously expressed cytoplasmic and nuclear protein with a predicted molecular weight of 42 kDa. Ataxin-3 functions as a deubiquitinating enzyme. Human Ataxin-3 shares 87% and 86% aa sequence identity with mouse and rat Ataxin-3, respectively. Full-length Ataxin-3 contains an N-terminal Josephin domain, two Ubiquitin interacting motifs, and a variable C-terminus consisting of a polyglutamine stretch and tail. As a deubiquitinating enzyme, Ataxin-3 plays a critical role in affecting the ubiquitination status of proteins for quality control and other cellular pathways. In turn, the ubiquitination of Ataxin-3 is was shown to enhance its capacity to cleave Ubiquitin chains. By opposing the actions of the Ubiquitin-conjugating (E2) enzyme UbE2W, Ataxin-3 is believed to control the activity of the Ubiquitin ligase (E3) C-terminus of Hsc70 Interacting Protein (CHIP). CHIP binds to protein chaperones and represents an important molecular link between the chaperone and Ubiquitin-proteasome system. Expression of murine Ataxin-3 is thought to be important for myogenesis, an effect that is dependent on the regulation of Integrin alpha 5 levels. Mutations in the ATXN3 gene are the cause of MJD, also known as spinocerebellar ataxia 3, an autosomal dominant neurodegenerative disorder characterized by nuclear aggregation of Ataxin-3 molecules featuring an expanded polyglutamine tract.