金山科研平台，产品价格货期咨询微信：jinshanbio Source:E. coli-derived, Gln25-Pro659, with an N-terminal Met and 6-His tag Accession #:AAB18278 N-terminal Sequence Analysis:Met Purity:>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 µg per lane. Endotoxin Level: Predicted Molecular Mass:74 kDa SDS-PAGE:66 kDa, reducing conditions Activity:Measured by its ability to liberate oligosaccharides from heparin.The specific activity is >3,000 pmol/min/µg, as measured under the described conditions. Formulation:Supplied as a 0.2 µm filtered solution in Tris, NaCl and Glycerol.See Certificate of Analysis for details. Molecule Information: Heparinase III Entrez Gene IDs: 8254931 (P. heparinus) Background: Heparinase III Heparan sulfate is a sulfated glycosaminoglycan with the repeating disaccharide units of -4HexA1,4GlcNAc beta1-. It is usually attached to the protein cores of proteoglycans found on the cell membrane and in the extracellular matrix where it binds to a variety of protein ligands and regulates a wide range of biological activities. Heparan sulfate has a domain structure where sulfated regions are interspaced with less or non-sulfated regions. Heparin shares the backbone structure with heparan sulfate but contains no non-sulfated regions. Heparinases are a family of lyases that release unsaturated oligosaccharides from heparin and heparan sulfate upon digestion. Heparinase I recognizes highly sulfated regions and is more specific for heparin. Heparinase II digests both heparin and heparan sulfate. Heparinase III prefers less-sulfated regions and is more active on heparan sulfate.