金山科研平台，产品价格货期咨询微信：jinshanbio Source:Mouse myeloma cell line, NS0-derived, Gln26-Glu370, with an N-terminal 6-His tag Accession #:O60507 N-terminal Sequence Analysis:His Purity:>95%, by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin Level: Predicted Molecular Mass:40 kDa SDS-PAGE:40-50 kDa, reducing conditions Activity:Measured by its ability to transfer sulfate from PAPS to PSGL-1 peptide (Gln-Ala-Thr-Glu-Tyr-Glu-Tyr-Leu-Asp-Tyr-Asp-Phe-Leu-Pro-Glu-Thr)The specific activity is >5 pmol/min/µg, as measured under the described conditions. Formulation:Supplied as a 0.2 µm filtered solution in Tris and NaCl.See Certificate of Analysis for details. Molecule Information: Tyrosylprotein Sulfotransferase 1/TPST1 Aliases: Tyrosylprotein Sulfotransferase 1 Entrez Gene IDs: 8460 (Human); 22021 (Mouse); 288617 (Rat) Background: TPST1 Tyrosine O-sulfation is a posttranslational modification of proteins in all multicellular organisms. This reaction is mediated by a Golgi enzyme activity called Tyrosylprotein Sulfotransferase (TPST) that transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to tyrosine residues within acidic motifs of polypeptides to form tyrosine O-sulfate ester. More than 60 proteins have been identified to be tyrosine sulfated, for some of which the functions have been identified. For example, sulfation of tyrosine residues in the leukocyte adhesion molecule P-selectin glycoprotein ligand 1 (PSGL-1) is required for binding to P-selectin on activated endothelium.