金山科研平台，产品价格货期咨询微信：jinshanbio Source:Chinese Hamster Ovary cell line, CHO-derived, Ser19-Lys352 Accession #:NP_149122 N-terminal Sequence Analysis:Ser19 Purity:>90%, by SDS-PAGE with silver staining. Predicted Molecular Mass:37.4 kDa SDS-PAGE:40 kDa, reducing conditions Activity:Measured by its ability to induce alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells.The ED₅₀ for this effect is typically 1.5-7.5 ng/mL.Measured by its ability to induce activation of beta -catenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells.Optimal concentrations should be determined by each laboratory for each application.Protein concentrations should be titrated based on cell type and if appropriate, passage number of the cell line. Molecule Information: Wnt-3a Long Name: Wingless-type MMTV Integration Site Family, Member 3a Entrez Gene IDs: 89780 (Human); 22416 (Mouse) Background: Wnt-3a

Wnt-3a is one of about 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved, cysteine-rich secreted glycoproteins important for normal developmental processes. Wnts bind to receptors of the Frizzled family in conjunction with a coreceptor of the low-density lipoprotein receptor-related protein family (LRP-5 or -6), or the Ryk atypical receptor tyrosine kinase. During development, Wnt-3a is a morphogen that is thought to coordinate somitogenesis and mesoderm boundary determination. When Wnt-3a is deleted, mice fail to develop a hippocampus, and show defects in anterior-posterior patterning, somite development and tailbud formation. Wnt-3a has also been implicated in chondrocyte differentiation.

Like other Wnts, Wnt-3a is modified by palmitate addition (at Cys 77) following glycosylation, which increases its hydrophobicity, secretion and activity. A second site at Ser 209 modified by palmitoleic acid also contributes. Human Wnt-3a shares 96% amino acid (aa) identity with mouse, bovine and canine Wnt-3a, and 89%, 86% and 84% aa identity with chicken, Xenopus and zebrafish Wnt-3a, respectively. It also shares 87% aa identity with Wnt-3. Human Wnt-3a is a 44 kDa secreted hydrophobic glycoprotein containing a conserved pattern of 24 cysteine residues.