金山科研平台，产品价格货期咨询微信：jinshanbio Source:Mouse myeloma cell line, NS0-derived, Thr95-Gly281, with an N-terminal Met and 6-His tag Accession #:P50591 N-terminal Sequence Analysis:Met Structure:Noncovalently-linked homotrimer and disulfide-linked homodimer Purity:>97%, by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin Level: Predicted Molecular Mass:21 kDa (monomer) SDS-PAGE:24 kDa, reducing conditions Activity:Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycinD. Matthews,N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221.The ED₅₀ for this effect is typically 4 - 12 ng/mL. The apoptotic effect of soluble trimeric rhTRAIL can be enhanced by oligimerization of the soluble rhTRAIL through the use of a cross-linking antibody (mouse anti-6-histidine, Catalog#MAB050). The ED₅₀ for this effect is typically 1 - 4 ng/mL. Note: This is one of multiple forms available for this protein. Check R&D Systems' website, www.RnDSystems.com, for a complete listing of the variants. Molecule Information: TRAIL/TNFSF10 Long Name: TNF-related Apoptosis-inducing Ligand Aliases: CD253; TNFSF10 Entrez Gene IDs: 8743 (Human); 22035 (Mouse) Background: TRAIL/TNFSF10 TNF-related apoptosis-inducing ligand (TRAIL), also known as Apo-2 ligand and TNFSF10, is a type II transmembrane protein with a carboxy-terminal extracellular domain that exhibits homology to other TNF superfamily members. Among TNF superfamily members, TRAIL is the most homologous to Fas Ligand, sharing 28% amino acid sequence identity in their extracellular domains. Human TRAIL shares 65% amino acid sequence identity with mouse TRAIL and is active on mouse cells.