货号：07-1458

品牌：Merck Millipore

规格：100Ug

目录价：￥5207.00

市场价格：￥4425.95

会员价格：￥4165.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-ROCK-1/2 Antibody | 07-1458 View All» Replaces: AB3885 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes ROCK-1 and ROCK-2. View All» Molecular Weight: 158 kDa View All» Immunogen: Synthetic peptide corresponding to human ROCK-1, 90.5% homology with ROCK-2 View All» Background Information: Rho-Associated Kinase (Rho-Kinase or ROK) has been classified into two isoforms: ROKα/ROCK-II and ROKβ/ROCK-I. These large cellular proteins (150-160 kDa), have been reported to be involved in the formation of stress fibers, focal adhesions and cardiac hypertonic responses. Current research suggests that ROK of smooth muscle may be involved in the contractile process via phosphorylation of MYPT1 and Myosin. The serine-threonine kinase ROCK-1 is a down-stream effector of Rho, and is involved in signal transduction pathways that regulate cell morphology, motility, and tumor progression through the reorganization of the actin cytoskeleton. View All» Species Reactivity:

• Human

• Mouse
  View All» Species Reactivity Note: Reacts with human and mouse. View All» Control: A431 cell lysate View All» Quality Assurance: Evaluated by Western Blot in A431 cell lysate.Western Blot Analysis: 1:500 dilution of this antibody detected ROCK-1/2 on 10 µg of A431 cell lysate. View All» Purification Method: Affinity Purfied View All» Presentation: Affinity purified rabbit serum in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. View All» Storage Conditions: Maintain at 2-8°C in undiluted aliquots for up to 1 year after date of receipt. View All» UniProt Number: Q13464 View All» Entrez Gene Number: NP_005397.1 View All» Gene Symbol:
  • EC 2.7.11.1

  • MGC131603

  • MGC43611

  • OTTHUMP00000162514

  • P160ROCK

  • PRO0435

  • p160-ROCK

  • p160ROCK
    View All» Alternate Names:
    • Renal carcinoma antigen NY-REN-35

    • Rho kinase

    • Rho-associated, coiled-coil containing protein kinase 1

    • Rho-associated, coiled-coil-containing protein kinase 1

    • p160 ROCK-1
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications: Western Blotting View All» Entrez Gene Summary: This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. View All» UniProt Summary: FUNCTION: Phosphorylates and activates DAPK3, which then regulates myosin light chain phosphatase through phosphorylation of MYPT1 thereby regulating the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. Required for centromere positioning and centromere-dependent exit from mitosis. Necessary for apoptotic membrane blebbing. CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.COFACTOR: Magnesium.ENZYME REGULATION: Activated by RHOA binding.SUBUNIT STRUCTURE: Binds RHOA (activated by GTP). Interacts with ADD1, GEM, RHOB, RHOC, MYLC2B and VIM By similarity. Binds RHOE, PPP1R12A, LIMK1 and LIMK2. Interacts with TSG101. Interacts with DAPK3. SUBCELLULAR LOCATION: Cytoplasm. Cytoplasm › cytoskeleton › centrosome › centriole By similarity. Golgi apparatus membrane; Peripheral membrane protein. Note: Associated with the mother centriole and an intercentriolar linker By similarity. A small proportion is associated with Golgi membranes. TISSUE SPECIFICITY: Detected in blood platelets. DOMAIN: The C-terminal auto-inhibitory domain interferes with kinase activity. RHOA binding leads to a conformation change and activation of the kinase. Truncated ROCK1 is constitutively activated.PTM: Autophosphorylated on serine and threonine residues. Phosphorylated upon DNA damage, probably by ATM or ATR.Cleaved by caspase-3 during apoptosis. This leads to constitutive activation of the kinase and membrane blebbing.MISCELLANEOUS: Inhibited by Y-27632.SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.Contains 1 AGC-kinase C-terminal domain.Contains 1 PH domain.Contains 1 phorbol-ester/DAG-type zinc finger.Contains 1 protein kinase domain.Contains 1 REM (Hr1) repeat. View All» Product Name: Anti-ROCK-1/2 View All» Concentration: 1 mg/mL View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 µg View All» Format: Affinity Purified View All» Host: Rabbit View All»
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