货号：05-723

品牌：Merck Millipore

规格：200UL

目录价：￥5053.00

市场价格：￥4295.05

会员价格：￥4042.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-RhoE/Rnd3 Antibody, clone 4 | 05-723 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Upstate (Millipore) View All» Specificity: This antibody recognizes RhoE/Rnd3, Mr 29 kDa. Weakly cross-reacts with Rnd1, Mr 27 kDa. View All» Molecular Weight: RhoE/Rnd3 (~29 kDa); Rnd1 (~27 kDa). View All» Immunogen: Full length fusion protein corresponding to residues 1-244 of mouse RhoE/Rnd3. Clone 4. View All» Clone: 4 View All» Isotype: IgG View All» Background Information: RhoE, also known as Rnd3, (Rho family GTPase 3) is a small G-protein that is a member of the Rnd family of Rho GTPases, which is a part of the Ras superfamily. The activity of Rnd1, Rnd2, and Rnd3/RhoE are not activated by the traditional conformational change of the GTP binding like other Rho family members. As the name dictates, Rnd proteins were associated with making a cell “round”. Like other small G-proteins, the do bind GTP, but unlike other G-proteins, they do not hydrolyze GTP to GDP. The actions of RhoE are associated with the actin cytoskeletal organization in response to external stimuli. RhoE acts antagonistically to RhoA in inducing loss of stress fiber formation, in part by binding to the RhoA downstream kinase Rho-associated kinase (ROCK1). This prevents ROCK1 activation and inhibits downstream signaling. RhoE does go through the post-translation modification of farnylation which enables it to localize and bind to various internal membranes. RhoE is also known to effect cycle progression. View All» Species Reactivity:

• Human

• Mouse
  View All» Species Reactivity Note: Mouse and human. View All» Application Notes: Immunocytochemistry: A 1:50 dilution of this antibody has been reported by an independent laboratory to show positive immunostaining for RhoE/Rnd3 in 3T3/A31 cells fixed with paraformaldehyde and permeabilized by Triton X-100 (Riento, K., 2003). View All» Control: Included Positive Antigen Control: Catalog #12-305, 3T3/A31 cell lysate. Add 2.5 µL of 2-mercaptoethanol/100 µL of lysate and boil for 5 minutes to reduce the preparation. Load 20 µg of reduced lysate per lane for minigels. View All» Quality Assurance: Routinely evaluated by western blot on RIPA lysates from 3T3/A31 cells.Western Blot Analysis: A 1:500-1:2000 dilution of this lot detected RhoE/Rnd3 in RIPA lysates from 3T3/A31 cells. View All» Purification Method: Unpurified View All» Presentation: Mouse monoclonal ascites IgG in buffer containing 0.05% sodium azide before the addition of glycerol to 30%. Liquid at -20ºC. View All» Storage Conditions: Stable for 1 year at -20°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap. View All» UniProt Number: P61587 View All» Entrez Gene Number: NM_028810 View All» Gene Symbol:
  • RND3

  • RHOE

  • RhoE

  • Rho8

  • memB

  • ARHE

  • RHO8

  • Rnd3
    View All» Alternate Names:
    • MemB protein

    • Rho family GTPase 3

    • ras homolog gene family, member E

    • small GTP binding protein Rho8
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Immunocytochemistry

      • Western Blotting
        View All» Entrez Gene Summary: Members of the Rho family of Ras-related GTPases, such as ARHE, regulate the organization of the actin cytoskeleton in response to extracellular growth factors. Like Ras (MIM 190020), Rho family members appear to cycle between an inactive GDP-bound form and an active GTP-bound form. Three major regulators of Rho activity have been identified: RhoGDIs, which interact with the GDP-bound Rho proteins to keep them in a resting complex (see MIM 601925); GEFs, which promote GDP/GTP exchange leading to activation of Rho proteins (see MIM 601855); and GAPs, which stimulate GTP hydrolysis and return the activated Rho protein to its inactive form (see MIM 602680) (Nobes et al., 1998 [PubMed 9531558]).[supplied by OMIM View All» UniProt Summary: FUNCTION: SwissProt: P61587 # Binds GTP but lacks intrinsic GTPase activity and is resistant to Rho-specific GTPase-activating proteins.SIZE: 244 amino acids; 27368 Da SUBUNIT: Binds ROCK1 (By similarity). Interacts with UBXD5.SUBCELLULAR LOCATION: Golgi apparatus membrane; Peripheral membrane protein.TISSUE SPECIFICITY: Ubiquitous.SIMILARITY: SwissProt: P61587 ## Belongs to the small GTPase superfamily. Rho family. View All» Brand Family: Upstate View All» Product Name: Anti-RhoE/Rnd3 Antibody, clone 4 | 05-723 View All» Concentration: Variable View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 200 µL View All» Format: Ascites View All» Host: Mouse View All»
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