货号：05-611

品牌：Merck Millipore

规格：100Ug

目录价：￥6601.00

市场价格：￥5610.85

会员价格：￥5280.80

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-phospho-VASP (Ser239) Antibody, clone 16C2 | 05-611 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Upstate (Millipore) View All» Specificity: phosphorylated VASP View All» Molecular Weight: 50kDa View All» Immunogen: phospho-peptide corresponding to amino acids 236-242 of human phospho-VASP (RKV[pS]KQE) View All» Modifications: Phosphorylation View All» Clone: 16C2 View All» Isotype: IgG1κ View All» Species Reactivity:

• Human

• Mouse

• Rat
  View All» Species Reactivity Note: Based on sequence homology this antibody is also predicted to cross-react with dog and cow. View All» Quality Assurance: routinely evaluated by immunoblot on RIPA lysates from CTLL cells View All» Purification Method: Thiophilic adsorption chromatography View All» Presentation: 100µg of mouse IgG1 from serum-free cell culture supernatant, purified by thiophilic adsorption and size exclusion chromatography. Lyophilized from 1ml of PBS, 0.1% sodium azide, PEG and sucrose. Lyophilized powder. Reconstitute with 100 µL H2O (15 min, RT) for a final concentration of 1 mg/mL. Store at -20°C. View All» Storage Conditions: 2 years at -20°C View All» UniProt Number: P50552 View All» Entrez Gene Number: NM_003370 View All» Gene Symbol: VASP View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
  • Flow Cytometry

  • Western Blotting

  • Immunocytochemistry
    View All» Entrez Gene Summary: Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG. View All» UniProt Summary: FUNCTION: SwissProt: P50552 # Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance and lamellipodial and filopodial dynamics in migrating cells. VASP promotes actin nucleation and increases the rate of actin polymerization in the presence of capping protein. Plays a role in actin-based activity of Listeria monocytogenes in platelets (By similarity).SIZE: 380 amino acids; 39830 Da SUBUNIT: Homotetramer. Interacts with PFN1, PFN2, LPP, ACTN1 and ACTG1. Interacts, via the EVH1, with the Pro-rich regions of ZYX. This interaction is important for targeting to focal adhesions and the formation of actin-rich structures at the apical surface of cells. Interacts, via the EVH1 domain, with the Pro-rich domain of Listeria monocytogenes actA. Interacts with APBB1IP. Interacts, via the Pro-rich domain, with the C-terminal SH3 domain of DNMBP (By similarity).SUBCELLULAR LOCATION: Cytoplasm. Cytoplasm, cytoskeleton. Cell junction, focal adhesion. Cell projection, lamellipodium membrane. Cell projection, filopodium membrane. Note=Targeted to stress fibers and focal adhesions through interaction with a number of proteins including MRL family members. Localizes to the plasma membrane in protruding lamellipodia and filopodial tips. Stimulation by thrombin or PMA, also translocates VASP to focal adhesions.TISSUE SPECIFICITY: Highly expressed in platelets.DOMAIN: "SwissProt: P50552 The EVH2 domain is comprised of 3 regions. Block A is a thymosin-like domain required for G-actin binding. The KLKR motif within this block is essential for the G-actin binding and for actin polymerization. Block B is required for F-actin binding and subcellular location, and Block C for tetramerization.PTM: Major substrate for cAMP-dependent (PKA) and cGMP-dependent protein kinase (PKG) in platelets. The preferred site for PKA is Ser-157, the preferred site for PKG, Ser-239. In ADP-activated platelets, phosphorylation by PKA or PKG on Ser-157 leads to fibrinogen receptor inhibition. Phosphorylation on Thr-278 requires prior phosphorylation on Ser-157 and Ser-239. In response to phorbol ester (PMA) stimulation, phosphorylated by PKC/PRKCA. In response to thrombin, phosphorylated by both PKC and ROCK1.SIMILARITY: Belongs to the Ena/VASP family. & Contains 1 WH1 domain. " View All» Brand Family: Upstate View All» Product Name: Anti-phospho-VASP (Ser239) Antibody, clone 16C2 | 05-611 View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µg View All» Format: Purified View All» Host: Mouse View All»
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