货号：MABT100

品牌：Merck Millipore

规格：100UL

目录价：￥5359.00

市场价格：￥4555.15

会员价格：￥4287.20

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Girdin, C-terminus Antibody, clone 10E6.1 | MABT100 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Molecular Weight: ~216 kDa observed View All» Immunogen: Recombinant protein corresponding to human Girdin. View All» Clone: 10E6.1 View All» Isotype: IgG1κ View All» Background Information: Girdin (Ccdc88a; APE, GIV, or HkRP1) is a ubiquitous protein belonging to the CCDC88 family. Girdin is an actin-binding protein which interacts with actin via its C-terminal domain, and may play a crucial role in cell motility and cytoskeleton remodeling. These effects may be regulated by a reciprocal relationship between Girdin and Akt. Akt phosphorylates Girdin, and Girdin binds to Akt and enhances phosphorylation of Akt, thereby promoting phosphorylation of Akt’s downstream targets such as GSK-3β and FOXO1A. Girdin/Akt signaling may play an important role in cancer by facilitating the proliferation of cancer cells. View All» Species Reactivity: Human View All» Species Reactivity Note: Demonstrated to react with Human. View All» Application Notes: Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Girdin in epithelial cells of human prostate tissue.Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Girdin in HeLa cells.Immunoprecipitation Analysis: A representative lot immunoprecipitated Girdin in COS-7 cell lysate. View All» Control: Human brain tissue lysate View All» Quality Assurance: Evaluated by Western Blot in human brain tissue lysate.Wesetern Blot Analysis: A 1:5000 dilution of this antibody detected Girdin in 10 µg of human brain tissue lysate. View All» Purification Method: Protein G purified View All» Presentation: Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. View All» Storage Conditions: Stable for 1 year at 2-8°C from date of receipt. View All» UniProt Number: Q3V6T2 View All» Entrez Gene Number: NP_001129069 View All» Gene Symbol:

• CCDC88A

• APE

• GRDN
  View All» Alternate Names:
  • Girdin

  • Akt phosphorylation enhancer

  • Coiled-coil domain-containing protein 88A

  • G alpha-interacting vesicle-associated protein

  • GIV

  • Girders of actin filament, Hook-related protein 1

  • HkRP1
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Western Blotting

    • Immunohistochemistry (Paraffin)

    • Immunocytochemistry

    • Immunoprecipitation
      View All» Entrez Gene Summary: This gene encodes a member of the Girdin family of coiled-coil domain containing proteins. The encoded protein is an actin-binding protein that is activated by the serine/threonine kinase Akt and plays a role in cytoskeleton remodeling and cell migration. The encoded protein also enhances Akt signaling by mediating phosphoinositide 3-kinase (PI3K)-dependent activation of Akt by growth factor receptor tyrosine kinases and G protein-coupled receptors. Increased expression of this gene and phosphorylation of the encoded protein may play a role in cancer metastasis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. View All» UniProt Summary: FUNCTION: Plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. Enhances phosphoinositide 3-kinase (PI3K)-dependent phosphorylation and kinase activity of AKT1/PKB, but does not possess kinase activity itself. Phosphorylation of AKT1/PKB thereby induces the phosphorylation of downstream effectors GSK3 and FOXO1/FKHR, and regulates DNA replication and cell proliferation. Essential for the integrity of the actin cytoskeleton and for cell migration. Required for formation of actin stress fibers and lamellipodia. May be involved in membrane sorting in the early endosome.SUBUNIT STRUCTURE: Interacts (via C-terminus) with DISC1; the interaction is direct. Interacts with AKT proteins; the interaction is inhibited in presence of DISC1. Homodimer. The non-phosphorylated form interacts with phosphatidylinositol 4-phosphate [PI4P] and weakly with phosphatidylinositol 3-phosphate [PI3P]. Interacts with microtubules. Interacts with actin through its C-terminal domain. Interacts with the C-terminus of AKT1/PKB.SUBCELLULAR LOCATION: Membrane. Cell membrane. Cytoplasm > cytosol. Cytoplasmic vesicle. Cell projection > lamellipodium. Note: Localizes to the cell membrane through interaction with phosphoinositides.TISSUE SPECIFICITY: Expressed ubiquitously.POST-TRANSLATIONAL MODIFICATION: Phosphorylation is induced by epidermal growth factor (EGF) in a phosphoinositide 3-kinase (PI3K)-dependent manner. Phosphorylation by AKT1/PKB is necessary for the delocalization from the cell membrane and for cell migration.SEQUENCE SIMILARITIES: Belongs to the CCDC88 family.SEQUENCE CAUTION: The sequence AAY14932.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence CAD97945.1 differs from that shown. Reason: Intron retention at the C-terminus. The sequence CAI46020.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. View All» Product Name: Anti-Girdin, C-terminus, clone 10E6.1 View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Purified View All» Host: Mouse View All»
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