货号：MABS10

品牌：Merck Millipore

规格：

目录价：￥4502.00

市场价格：￥3826.70

会员价格：￥3601.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-NEDD8-conjugating enzyme Ubc12 Antibody, clone 9H1.1 | MABS10 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Molecular Weight: ~ 21 kDa observed View All» Epitope: 0 View All» Immunogen: GST-tagged recombinant protein corresponding to human NEDD8-conjugating enzyme Ubc12. View All» Clone: 9H1.1 View All» Isotype: IgG1κ View All» Background Information: NEDD8-conjugating enzyme Ubc12, an E2 enzyme necessary for NEDDylation, has been shown to form a thioester linkage with the ubiquitin like protein NEDD8 in the presence of the NEDD8 activating enzyme complex Uba3/APP-BP1. The NEDD8 pathway is essential for cell cycle progression and morphogenesis. View All» Species Reactivity:

• Mouse

• Human
  View All» Species Reactivity Note: Demonstrated to react with mouse and human. View All» Application Notes: Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected NEDD8-conjugating enzyme Ubc12 in NIH/3T3, A431, and HeLa cells. View All» Control: NIH/3T3 cell lysate View All» Quality Assurance: Evaluated by Western Blot in NIH/3T3 cell lysate.Western Blot Analysis: 1 µg/mL of this antibody detected NEDD8-conjugating enzyme Ubc12 on 10 µg of NIH/3T3 cell lysate. View All» Purification Method: Protein G View All» Presentation: Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. View All» Storage Conditions: Stable for 1 year at 2-8°C from date of receipt. View All» UniProt Number: P61081 View All» Entrez Gene Number: NP_003960 View All» Gene Symbol:
  • UBE2M

  • UBC12
    View All» Alternate Names:
    • neural precursor cell expressed

    • NEDD8 carrier protein

    • NEDD8 protein ligase

    • Ubiquitin-conjugating enzyme E2 M
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Western Blotting

      • Immunocytochemistry
        View All» Entrez Gene Summary: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin. [provided by RefSeq]. View All» UniProt Summary: FUNCTION: Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation. CATALYTIC ACTIVITY: ATP + NEDD8 + protein lysine = AMP + diphosphate + protein N-NEDD8yllysine.PATHWAY: Protein modification; protein neddylation.SUBUNIT STRUCTURE: Interacts with UBA3 and RBX1. DOMAIN: Both the N-terminal docking peptide and the catalytic core domain must bind the UBA3-NAE1 complex simultaneously for optimal transfer of NEDD8.SEQUENCE SIMILARITIES: Belongs to the ubiquitin-conjugating enzyme family. UBC12 subfamily. View All» Product Name: Anti-NEDD8-conjugating enzyme Ubc12, clone 9H1.1 View All» Concentration: 1.0 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Purified View All» Host: Mouse View All»
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