货号:MAB4395
品牌:Merck Millipore
规格:EA
目录价:¥3779.00
市场价格:¥3212.15
会员价格:¥3023.20
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Description:
hPlurES-1 Antibody, clone IH3 | MAB4395
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Promotional Text:
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Specificity:
This antibody reacts with a cell surface epitope of a 65 kDa protein found to be expressed on pluripotent human embryonic stem cells.
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Molecular Weight:
65 kDa (major) and ~23 kDa (minor)
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Immunogen:
Human Embryonic Stem Cells (hESCs)
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Clone:
IH3
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Isotype:
IgG1
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Background Information:
Human embryonic stem cells (hESCs) have been traditionally characterized using a panel of antibodies to cell surface markers, such as SSEA-3, TRA-1-60, and TRA-1-81; however, none of these traditional markers are entirely specific to hES cells (Laslett et al., 2003). Therefore, there is a continuous quest to not only identify, but also gain a better understanding of new pluripotency markers to better characterize the human embryonic stem cell populations of interest. The novel hPlurES-1 Antibody is a monoclonal antibody that has been shown to identify a 65 kDa antigen that is expressed on the cell surface of human embryonic stem cells. This antibody recognizes a protein epitope that appears to be expressed within a subset of Oct-4-positive human embryonic stem cell populations, as indicated by double-staining analysis using flow cytometry and immunocytochemistry.
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Species Reactivity:
Human
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Species Reactivity Note:
Reacts with human. Reactivity to other species has not yet been determined.
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Application Notes:
Immunocytochemistry: Recommended working dilution is 1:50.Western Blotting: Recommended working dilution is 1.5 µg/mL.Flow Cytometry: Recommended working dilution is 25 µg/mL for 1 x 106 cells.
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Purification Method:
Protein G sepharose column
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Presentation:
Purified mouse monoclonal IgG1 in a preservative-free buffer containing PBS, pH 7.4.
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Storage Conditions:
Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months. (Note: this product is stored in a preservative-free buffer, and should be handled under aseptic conditions).
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
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Western Blotting
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Flow Cytometry
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Immunocytochemistry
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Product Name:
hPlurES-1 Antibody, clone IH3
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Concentration:
1 mg/mL
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Antibody Type:
Monoclonal Antibody
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Qty/Pk:
100 μg
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Format:
Purified
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Host:
Mouse
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