货号：04-1005

品牌：Merck Millipore

规格：

目录价：￥3639.00

市场价格：￥3093.15

会员价格：￥2911.20

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-FKHR Antibody, clone EP927Y, Rabbit | 04-1005 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes both phosphorylated and unphosphorylated FKHR. View All» Molecular Weight: ~80 kDa View All» Epitope: Ser329 View All» Immunogen: Synthetic peptide corresponding to residues surrounding Ser329 of human FKHR. View All» Clone: EP927Y View All» Isotype: IgG View All» Background Information: The Non-Radioactive FoxO1 Transcription Factor Assay kit is provided in a 96-well format. During the assay, the Capture Probe, a double stranded biotinylated oligonucleotide containing the consensus sequence for FoxO1 binding (5'-TTGTTTAC-3'), is mixed with whole cell or nuclear extract in the Transcription Factor Assay Buffer provided directly in the Streptavidin coated plate. When incubated together, FoxO1 contained in the nuclear extract binds to its consensus sequence. The biotinylated double stranded oligonucleotide bound to FoxO1 protein is immobilized and any inactive, unbound material is washed away. The bound FoxO1 transcription factor is detected with a specific rabbit anti-FoxO1, primary antibody. An HRP-conjugated secondary antibody is then used for detection and provides sensitive colorimetric detection that can be read in a spectrophotometric plate reader. Included in the kit are positive control whole cell extract, a non-specific double stranded oligonucleotide, and a specific double stranded competitor oligonucleotide. View All» Species Reactivity:

• Human

• Mouse

• Rat
  View All» Species Reactivity Note: Human, mouse, and rat. View All» Application Notes: Immunohistochemistry Analysis: A 1:250-500 dilution from a previous lot detected FKHR in human bladder carcinoma tissue.Immunocytochemistry Analysis: A 1:100-250 dilution from a previous lot detected FKHR in HeLa cells. View All» Control: NIH/3T3 cell lysate View All» Quality Assurance: Evaluated by Western Blot on NIH/3T3 cell lysates.Western Blot Analysis: A 1:500-1,000 dilution of this antibody was used to detect FKHR in NIH/3T3 cell lysate. View All» Purification Method: Unpurified View All» Presentation: Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA. View All» Storage Conditions: Stable for 1 year at -20ºC from date of receipt.Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. View All» UniProt Number: Q12778 View All» Entrez Gene Number: NM_002015.3 View All» Gene Symbol:
  • FKH1

  • FKHR

  • FOXO1A

  • OTTHUMP00000018301
    View All» Alternate Names:
    • Forkhead box protein O1A

    • Forkhead in rhabdomyosarcoma

    • forkhead box O1

    • forkhead box O1A (rhabdomyosarcoma)

    • forkhead homolog in rhabdomyosarcoma

    • forkhead, Drosophila, homolog of, in rhabdomyosarcoma
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Western Blotting

      • Immunocytochemistry

      • Immunohistochemistry (Paraffin)
        View All» UniProt Summary: FUNCTION: Transcription factor. Ref.9SUBUNIT STRUCTURE: Interacts with LRPPRC By similarity.SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note: Shuttles between cytoplasm and nucleus. TISSUE SPECIFICITY: Ubiquitous. PTM: Phosphorylated by AKT1; insulin-induced By similarity. IGF1 rapidly induces phosphorylation of Ser-256, Thr-24, and Ser-319. Phosphorylation of Ser-256 decreases DNA-binding activity and promotes the phosphorylation of Thr-24, and Ser-319, permitting phosphorylation of Ser-322 and Ser-325, probably by CK1, leading to nuclear exclusion and loss of function. Phosphorylation of Ser-329 is independent of IGF1 and leads to reduced function. Phosphorylated upon DNA damage, probably by ATM or ATR.INVOLVEMENT IN DISEASE: Chromosomal aberrations involving FOXO1 are a cause of rhabdomyosarcoma 2 (RMS2) [MIM:268220]; also known as alveolar rhabdomyosarcoma. Translocation (2;13)(q35;q14) with PAX3; translocation t(1;13)(p36;q14) with PAX7. The resulting protein is a transcriptional activator.SEQUENCE SIMILARITIES: Contains 1 fork-head DNA-binding domain. View All» Product Name: Anti-FKHR, clone EP927Y, Rabbit Monoclonal Antibody View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Unpurified View All» Host: Rabbit View All»
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