货号：03-251

品牌：Merck Millipore

规格：10assays

目录价：￥6533.00

市场价格：￥5553.05

会员价格：￥5226.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: RIPAb+™ IGF2BP2 Antibody | 03-251 View All» Promotional Text: Special Offer on Antibodies! Click Here! View All» Product Overview: RIPAb+ antibodies are evaluated using the RNA Binding Protein Immunoprecipitation (RIP) assay. Each RIPAb+ antibody set includes a negative control antibody to ensure specificity of the RIP reaction and is verified for the co-immunoprecipitation of RNA associated specifically with the immunoprecipitated RNA-binding protein of interest. Where appropriate, the RIPAb+ set also includes quantitative RT-PCR control primers (RIP Primers) to biologically validate your IP results by successfully co-precipitating the specific RNA targets, such as messenger RNAs. The qPCR protocol and primer sequences are provided, allowing researchers to validate RIP protocols when using the antibody in their experimental context. If a target specific assay is not provided, the RIPAb+ kit is validated using an automated microfluidics-based assay by enrichment of detectable RNA over control immunoprecipitation.IGF2BP2 (Insulin-like growth factor 2 mRNA-binding protein 2) is a member of the IGF-II mRNA-binding protein family that post-transcriptionally regulates IGF2 during developmental stages. IGF2BP1, 2, and 3 bind the UTR region of IGF2 transcripts, and are implicated in the transport of RNA targets, to enable protein synthesis at specific locations in the cytoplasm. Research has shown that the IGF2 gene may be involved in ovarian cancer. View All» Molecular Weight: ~66 kDa View All» Immunogen: Histidine-tagged recombinant protein corresponding to human IGF2BP2 View All» Isotype: IgG1κ View All» Background Information: Insulin-like growth factor 2 mRNA-binding protein 2 (IGF2BP2) is a member of the IGF-II mRNA binding protein family that posttranscriptionally regulates IGF2 during developmental stages. IGF2 mRNA-binding protein family members (IGF2BP1, 2 and 3) bind UTR region of IGF2 transcripts and are implicated in the transport of RNA targets to enable protein synthesis at specific locations in the cytoplasm. Studies have indicated variations in the IGF2BP2 gene may affect fetal glucose metabolism and plays a role in type 2 diabetes. In addition to playing a role in glucose metabolism and dieabetes, the insulin-like growth factor (IGF) signaling axis plays an important role in cancer biology. Research has shown IGF2 to be emerging as an important gene for ovarian cancer. View All» Species Reactivity: Human View All» Species Reactivity Note: Demonstrated to react in human View All» Application Notes: Western Blot Analysis: Representative lot data.HepG2 cell lysate was probed with Anti-IGF2BP2, clone 5E10-3E1 (1:20,000 dilution). Proteins were visualized using a Goat Anti-Mouse IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates IGF2BP2 (~66 kDa).Note: An uncharacterized band appears at ~50 kDa in some lysates. View All» Control: Includes normal mouse IgG and primers specific for human IGF2 mRNA. View All» Quality Assurance: RNA Binding Protein Immunoprecipitation:Representative lot dataRIP Lysate prepared from 293 cells (2 X 10E7 cell equivalents per IP) were subjected to immunoprecipitation using 1.25 µg of either a normal mouse IgG or 1.25 µg Anti-IGF2BP2 antibody and the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Cat. # 17-700).Successful immunoprecipitation of IGF2BP2-associated RNA was verified by qPCR using RIP Primers IGF2, (Figure 1). Please refer to the Magna RIP™ (Cat. # 17-700) or EZ-Magna RIP™ (Cat. # 17-701) protocol for experimental details. View All» Purification Method: Protein G purified View All» Presentation: Anti-IGF2BP2 (Mouse Monoclonal). One vial containing 12.5 µg protein G purified monoclonal in buffer containing 0.1 M Tris-glycine (pH 7.4), 150mM NaCl, 0.05% sodium azide before the addition of glycerol to 30%. Store at -20° C.Concentration: 0.176 mg/mLNormal mouse IgG. One vial containing 125 µg purified mouse IgG in 125 µL storage buffer containing 0.1% sodium azide. Store at -20°C.RIP Primer IGF2. One vial containing 75 μL of 5 μM of each primer specific for human IGF2 mRNA. Store at -20°C.FOR: GCG GCT TCT ACT TCA GCA GREV: CAG GTG TCA TAT TGG AAG AAC View All» Storage Conditions: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. View All» UniProt Number: Q9Y6M1 View All» Entrez Gene Number: NP_001007226 View All» Gene Symbol:

• IGF2BP2

• IMP2

• VICKZ2
  View All» Alternate Names:
  • Insulin-like growth factor 2 mRNA-binding protein 2

  • IGF2 mRNA-binding protein 2

  • IMP-2

  • Hepatocellular carcinoma autoantigen p62

  • IGF-II mRNA-binding protein 2

  • VICKZ family member 2
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Western Blotting

    • RNA Binding Protein Immunoprecipitation (RIP)
      View All» Entrez Gene Summary: This gene encodes a member of the IGF-II mRNA-binding protein (IMP) family. The protein encoded by this gene contains several four KH domains and two RRM domains. It functions by binding to the 5' UTR of the insulin-like growth factor 2 (IGF2) mRNA and regulating IGF2 translation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]. View All» UniProt Summary: FUNCTION: Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binding is isoform-specific. May regulate translation of target mRNAs. SUBUNIT STRUCTURE: Interacts with HNRPD and IGF2BP1.SUBCELLULAR LOCATION: Cytoplasm. Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Localizes at the connecting piece and the tail of the spermatozoa. TISSUE SPECIFICITY: Expressed in oocytes, granulosa cells of small and growing follicles, Leydig cells, spermatogonia and semen (at protein level). Expressed in testicular cancer (at protein level). Expressed weakly in heart, placenta, skeletal muscle, bone marrow, colon, kidney, salivary glands, testis and pancreas. Detected in fetal liver, fetal ovary, gonocytes and interstitial cells of the testis. DOMAIN: The third and fourth KH domains are important for binding to the untranslated region (UTR) of target mRNA. MISCELLANEOUS: Autoantibodies against IGF2BP2 are detected in sera from some patients with hepatocellular carcinoma.SEQUENCE SIMILARITIES: Belongs to the RRM IMP/VICKZ family.Contains 4 KH domains.Contains 2 RRM (RNA recognition motif) domains.SEQUENCE CAUTION: The sequence AAH21290.1 differs from that shown. Reason: Erroneous initiation. View All» Product Name: RIPAb+™ IGF2BP2 View All» Concentration: 0.176 mg/mL View All» Qty/Pk: 10 assays View All» Format: Purified View All» Host: Mouse View All» Packaging: 10 assays per set. Recommended use: 1.25 μg of antibody per RIP (dependent upon biological context). View All»
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