货号：07-2126

品牌：Merck Millipore

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金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-phospho-FOXO1 (Thr24) View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes FOXO1 phosphorylated at Thr24. View All» Molecular Weight: ~78 kDa observed.Calculated molecular weight is 70 kDa. FOXO1 has been shown to migrate to ~78 kDa in western blots (Kamei, Y., et al. (2004). JBC. 279(39):41114-41123). View All» Epitope: Phosphorylated Thr24 View All» Immunogen: KLH-conjugated linear peptide corresponding to human FOXO1 phosphorylated at Thr24. View All» Modifications: Phosphorylation View All» Background Information: Forkhead box O (FOXO) transcription factors play an important role in modulating metabolic functions and cell proliferation. Given the relatively high expression of FOXO1 in insulin-responsive tissues, this transcription factor is highly poised to regulate energy metabolism. When nutrient and insulin levels are low, FOXO1 promotes expression of gluconeogenic enzymes. Conversely, in the fed state, insulin levels rise and stimulate uptake of glucose primarily into skeletal muscle and other organs, including adipose tissue. Under certain pathophysiologic conditions, including insulin resistance, negative signaling to FOXO1 is compromised. Increased cell proliferation occurs when FOXO1 is inactivated when phosphorylated by Akt at Thr24. View All» Species Reactivity: Human View All» Species Reactivity Note: Tested and passed on Human. View All» Control: HEK293 cell lysate untreated and BMP2-treated View All» Quality Assurance: Evaluated by Western Blot in HEK293 cell lysate untreated and BMP2-treated.Western Blot Analysis: 2 µg/mL of this antibody detected phospho-FOXO1 (Thr24) in HEK293 cell lysate untreated and BMP2-treated. View All» Purification Method: Affinity Purified View All» Presentation: Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. View All» Storage Conditions: Stable for 1 year at 2-8°C from date of receipt. View All» UniProt Number: Q12778 View All» Entrez Gene Number: NP_002006 View All» Gene Symbol: FOXO1, FOXO1A, FKHR View All» Alternate Names: Forkhead box protein O1, Forkhead box protein O1A, Forkhead in rhabdomyosarcoma View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications: Western Blotting View All» UniProt Summary: Function: Transcription factor which acts as a regulator of cell responses to oxidative stress. In the presence of KIRT1, mediates down-regulation of cyclin D1 and up-regulation of CDKN1B levels which are required for cell transition from proliferative growth to quiescence.Subunit structure: Interacts with LRPPRC. Interacts with SIRT1 and this interaction requires the presence of KRIT1.Subcellular location: Cytoplasm. Nucleus. Note: Shuttles between cytoplasm and nucleus.Tissue specificity: Ubiquitous. Induction: Expression is regulated by KRIT1. Post-translational modification: Phosphorylated by AKT1; insulin-induced. IGF1 rapidly induces phosphorylation of Ser-256, Thr-24, and Ser-319. Phosphorylation of Ser-256 decreases DNA-binding activity and promotes the phosphorylation of Thr-24, and Ser-319, permitting phosphorylation of Ser-322 and Ser-325, probably by CK1, leading to nuclear exclusion and loss of function. Phosphorylation of Ser-329 is independent of IGF1 and leads to reduced function. Phosphorylated upon DNA damage, probably by ATM or ATR. Involvement in disease: Defects in FOXO1 are a cause of rhabdomyosarcoma type 2 (RMS2) [MIM:268220]. It is a form of rhabdomyosarcoma, a highly malignant tumor of striated muscle derived from primitive mesenchimal cells and exhibiting differentiation along rhabdomyoblastic lines. Rhabdomyosarcoma is one of the most frequently occurring soft tissue sarcomas and the most common in children. It occurs in four forms: alveolar, pleomorphic, embryonal and botryoidal rhabdomyosarcomas. Note=Chromosomal aberrations involving FOXO1 are found in rhabdomyosarcoma. Translocation (2;13)(q35;q14) with PAX3; translocation t(1;13)(p36;q14) with PAX7. The resulting protein is a transcriptional activator.Sequence similarities: Contains 1 fork-head DNA-binding domain. View All» Product Name: Anti-phospho-FOXO1 (Thr24) View All» Concentration: 1 mg/mL View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Affinity Purified View All» Host: Rabbit View All»