货号：X23985

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：10 X 50 UG

目录价：￥3755.00

市场价格：￥3379.50

会员价格：￥3379.50

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 Labeled calcium indicators are molecules that exhibit an increase in fluorescence upon binding Ca²⁺. They have uses in many calcium signaling investigations, including measuring Ca²⁺ in cells and tissues that have high levels of autofluorescence and also for detecting Ca²⁺ release generated by photoreceptors and photoactivatable chelators. Cells may be loaded with the AM ester forms of these calcium indicators by adding the dissolved indicator directly to dishes containing cultured cells. The fluorescence signal from these cells is generally measured using fluorescence microscopy.Calcium Indicator (AM Ester) Specifications:• Label (Ex/Em of Ca²⁺–bound form): X-rhod-5F (581/603 nm)• Fluorescence intensity increase upon binding Ca²⁺: >100 fold• K_d for Ca²⁺ in the absence of Mg²⁺, in buffer: ~1.6 µM• Exhibit fluorescence increase upon binding Ca²⁺ with little shift in wavelengthUsing TPEN to Control Heavy Metal Cations In addition, BAPTA-based indicators such as these bind various heavy metal cations (e.g., Mn²⁺, Zn²⁺, Pb²⁺) with substantially higher affinity than Ca²⁺. Perturbations to calcium measurements caused by presence of these ions can be controlled using the heavy metal-selective chelator TPEN.More Choices for Fluorescent Calcium Indicators We offer a large selection of Molecular Probes® calcium indicators for use in various experimental scenarios, for example dextran versions for reduced leakage and compartmentalization and BAPTA conjugates for detecting high-amplitude calcium transients. For more information, review Fluorescent Ca²⁺ Indicators Excited with Visible Light—Section 19.3 in the Molecular Probes® Handbook. For UV-excitable Ca²⁺ indicators, protein-based Ca²⁺ indicators, conjugates of Ca²⁺ indicators, and for fluorescence-based indicators of other metal ions (i.e., Mg²⁺, Zn²⁺) review Indicators for Ca²⁺, Mg²⁺, Zn²⁺ and Other Metal Ions—Chapter 19 in the Molecular Probes® Handbook. For Research Use Only. Not for human or animal therapeutic or diagnostic use. 详细说明 常用规格

Platform:	Fluorescence Microscope
Product Size:	10 x 50 µg
Indicator Type:	Calcium Indicator
Detection Method:	Fluorescent
Cell Permeability:	Cell-Permeant
Indicator Properties:	Fluorescent Dye-Based
Shipping Condition:	Room Temperature
Regulatory Statement:	For Research Use Only. Not for use in diagnostic procedures.

内容和储存

Store in freezer (-5 to -30°C) and protect from light.

化学结构 X-rhod-5F, AM 分子式 C₅₉H₆₄BrFN₄O₁₉分子量 1232.07 CAS名称/编号 N/A 荧光光谱 X-Rhod-5F (calcium bound)

Fluorescence Ex/Em spectra of Ca<sup>2+</sup>-saturated X-Rhod-5F in pH 7.4 buffer.

查看此光谱中的 数据点， 或在这里查看 Fluorescence SpectraViewer