货号：18009027

品牌：ThermoFisher /Invitrogen/ Gibco/ ABI

规格：1000 UN

目录价：￥643.00

市场价格：￥578.70

会员价格：￥578.70

金山科研平台，产品价格货期咨询微信：jinshanbio 描述 Calf Intestinal Alkaline Phosphatase (CIAP) is a phosphomonoesterase that removes 3´ and 5´ phosphates from DNA and RNA.Concentration: 1 unit/µLApplications: Dephosphorylation of 5´-phosphorylated termini of vector DNA to prevent self-ligation (1). Dephosphorylation of 5´ termini of nucleic acids prior to forward reaction with kinase.Source: Purified from calf intestinal mucosa.Performance and Quality Testing: Endodeoxyribonuclease, 3´ exodeoxyribonuclease, and ribonuclease assays; dephosphorylation efficiency measured in a transformation assay.Unit Definition: One unit hydrolyzes 1 µmol of 4-nitrophenyl phosphate in 1 min. at 37°C.Unit Reaction Conditions: 1 M diethanolamine buffer, 10 mM 4-nitrophenyl phosphate, 0.25 mM MgCl₂ (pH 9.8) in 900 µl for 10 min. at 37°C. 规格

Enzyme:	CIP
Product Size:	1,000 units
Shipping Condition:	Approved for shipment on Wet or Dry Ice

内容及储存 Calf Intestinal Alkaline Phosphatase (1 unit/µL) is supplied with a vial of 10X dephosphorylation buffer [500 mM Tris-HCl (pH 8.5), 1 mM EDTA], vial of dilution buffer [50% glycerol, 25 mM Tris-HCl (pH 7.6), 1 mM MgCl₂ , and 0.1 mM ZnCl₂ ]. Store Calf Intestinal Alkaline Phosphatase at -20°C.